|Table of Contents|

The differential expression and bioinformatics analysis of miR-424 in human esophageal squamous cell carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 04
Page:
557-562
Research Field:
Publishing date:

Info

Title:
The differential expression and bioinformatics analysis of miR-424 in human esophageal squamous cell carcinoma
Author(s):
Liu Qiao; Yao Li; Zhang Hongchen; Zhang Jiarui; Gong Li; Zhu Shaojun; Han Xiujuan; Lan Miao; Zhang Fuqin; Zhang Wei
Department of Pathology,Tangdu Hospital of Air Force Military Medical University,Shaanxi Xi' an 710038,China.
Keywords:
esophageal squamous cell carcinoma; has-miR-424; bioinformatics
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2020.04.008
Abstract:
Objective：To explore the hsa-miR-424 expression in the cell lines of human esophageal squamous cell carcinoma,predict and analyze the target genes of hsa-miR-424 using bioinformatic method and to provide theoretical guidance for the further function study.Methods:The relative expressions of hsa-miR-424 were detected by real-time quantitative PCR in human esophageal squamous cell carcinoma cell lines kyse520,kyse410 and human normal esophageal epithelial cell line Het-1A,and esophageal squamous cell carcinoma tissues and corresponding nomal esophageal tissues.hsa-miR-424 target genes were predicted by MIRDB,miRanda and Target Scan,then combined with the confirmed gene database DIANALAB-TarBase7.0 and miRTarBase.Functional annotation classification and pathway enrichment analysis based on GO and KEGG databases were performed on target gene sets predicted by miR-424 using the online database metascape.Results:Compared with normal esophageal epithelial cells,the expression levels of hsa-miR-424 in human esophageal squamous cell carcinoma cell lines kyse520,kyse410 were significantly higher.Compared with normal esophageal epithelial tissues,the expression levels of hsa-miR-424 in human esophageal squamous cell carcinoma tissues were significantly higher.About 77.8 percent (56/72) of the patients had a upregulation of more than 50 percent.hsa-miR-424 is highly conserved in species evolution.The function of the target genes mainly focused on regulating cell growth,proliferation and positive and negative regulation of apoptotic process,and participating in the biological processes of protein dephosphorylation and ubiquitination,histone modification and methylation,regulation of response to wounding (P＜0.01).hsa-miR-424 target genes were significantly enriched in the MAPK,p53 and tyrosine kinase signal pathway closely related with tumor (P＜0.01).Conclusion:The highly expression of hsa-miR-424 in esophageal squamous cell carcinoma suggesting that hsa-miR-424 may be involved in the regulation of pathogenic of esophageal squamous cell carcinoma.

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陕西省重点研发计划（编号：2017SF-184）
Last Update: 2020-01-14