|Table of Contents|

Effects of NFATc1 on proliferation and apoptosis of breast cancer cells by regulating RhoA/ROCK signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 04
Page:
562-568
Research Field:
Publishing date:

Info

Title:
Effects of NFATc1 on proliferation and apoptosis of breast cancer cells by regulating RhoA/ROCK signaling pathway
Author(s):
Liu Mengmeng; Wei Li; Wang Yijuan; Liu Peng; Liu Xinghua; Zhang Ming
Department of Pathology,the Third People's Hospital of Zhengzhou,Henan Zhengzhou 450000,China.
Keywords:
breast cancer; NFATc1; proliferation; apoptosis
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2020.04.009
Abstract:
Objective：To detect the expression of NFATc1 in breast cancer and adjacent tissues,and to explore its effect on the proliferation and apoptosis of breast cancer cells.Methods:Adjacent tissues and tumor cancers from thirty-nine breast cancer patients were collected from our hospital.RT-PCR,immunoblotting and immunohistochemical staining were used to detect the expression of NFATc1 in breast cancer tissues and adjacent tissues.Further,the construction of NFATc1 stable knockout MCF7 and MDA-MB-231 using small interfering RNA (siRNA) cell line.The effect of NFATc1 knockdown on the proliferation of breast cancer cells was detected by CCK-8 kit and clone formation assay.The ratio of Ki67 positive cells in breast cancer cells of control group and NFATc1 knockout group was detected by Ki67 staining.In addition,flow cytometry was used to detect the effect of NFATc1 gene knockdown on apoptosis of breast cancer cells.Finally,the expression of RhoA/ROCK signaling pathway in each group was analyzed by immunoblotting.Results:The mRNA and protein expression of NFATc1 in breast cancer patients was significantly increased (P＜0.05).Flow cytometry results showed that NFATc1 siRNA intervention significantly promoted apoptosis in two breast cancer cell lines (P＜0.05).In addition,Ki67 staining,CCK-8 assay and colony formation assay showed that the proliferative capacity of the two breast cancer cell lines was significantly decreased after NFATc1 knockout (P＜0.05).Western blot results revealed that NFATc1 knockdown significantly inhibited the activation of RhoA/ROCK signaling pathway in cancer cells (P＜0.05).Conclusion:Inhibition of NFATc1 can promote the apoptosis of breast cancer cells and inhibit the proliferation of breast cancer cells by inhibiting RhoA/ROCK signaling pathway in breast cancer cells.Therefore,NFATc1 is expected to become a new target for the treatment of breast cancer.

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河南省医学科技攻关计划（编号：170001021）
Last Update: 2020-01-14