|Table of Contents|

lncRNA XIST involved in the proliferation of esophagus cancer by the regulation of miR-486-5p

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 04
Page:
539-543
Research Field:
Publishing date:

Info

Title:
lncRNA XIST involved in the proliferation of esophagus cancer by the regulation of miR-486-5p
Author(s):
Wang Shuai1; Li Wenya2
1.Fuxin Central Hospital,Liaoning Fuxin 123000,China;2.First Hospital of China Medical University,Liaoning Shenyang 110000,China.
Keywords:
esophagus cancer; proliferation; lncRNA XIST; miR-486-5p
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2020.04.004
Abstract:
Objective：To evaluate the expression of lncRNA XIST in esophagus cancer tissues and investigate the effect of lncRNA XIST on the proliferation of esophagus cancer cells.Methods:The expression of lncRNA XIST in 23 pairs of esophagus cancer and adjacent tissue were determined by real-time PCR.The expression of miR-486-5p in three esophagus cancer cell lines was also detected.The two lncRNA XIST-high-expressed cells were transfected with lncRNA XIST siRNA or NC siRNA and the interfere efficient was determined by qRT-PCR.Cell proliferation activities were examined by CCK-8 assay.Dual-Luciferase reporter assay was also used to exam whether miR-486-5p could bind to lncRNA XIST.Moreover,the expression of miR-486-5p was also detected by qRT-PCR.Results:The expression of lncRNA XIST was significantly increased in esophagus cancer tissues compared with cancer-adjacent tissue (P＜0.01).Eca-109 and TE-1 cells showed the highest level of lncRNA XIST in the three esophagus cancer cell lines.Transfect with lncRNA XIST siRNA in Eca-109 and TE-1 cells reduced the level of lncRNA XIST to （75.50±0.89）% and （64.74±13.42）% respectively.Cell proliferation activity of Eca-109 and TE-1 cells was also significantly reduced by lncRNA XIST siRNA.Dual-Luciferase reporter assay showed that the relative luciferase activity was significantly increased in wt-XIST +miR-486-5p mimic group.Furthermore,the expression of miR-486-5p was also up-regulated by lncRNA XIST siRNA.Conclusion:lncRNA XIST is closely related to the occurrence and development of esophagus cancer,miR-486-5p may be a novel target in the prevention and treatment of esophagus cancer.

References:

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Memo

Memo:
辽宁省科学技术计划项目(编号：2015020561)
Last Update: 2020-01-14