|Table of Contents|

Effects of interferon-inducible protein-10 on proliferation,invasiveness and drug resistance of human diffuse large B-cell lymphoma cell lines

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 04
Page:
543-547
Research Field:
Publishing date:

Info

Title:
Effects of interferon-inducible protein-10 on proliferation,invasiveness and drug resistance of human diffuse large B-cell lymphoma cell lines
Author(s):
Su Zhixiang
Internal Medicine Department,Shaanxi Cancer Hospital,Shaanxi Xi'an 710061,China.
Keywords:
diffuse large B-cell lymphoma; interferon-inducible protein-10; SU-DHL-4 cells
PACS:
R733.4
DOI:
10.3969/j.issn.1672-4992.2020.04.005
Abstract:
Objective：To investigate the effect of interferon induced protein-10 (IP-10) on proliferation,migration,invasion and expression of drug resistance protein of human diffuse large B-cell lymphoma cell line SU-DHL-4.Methods:SU-DHL-4 cells were divided into control group and 3 experimental groups which were treated with IP-10 at different concentration (10 ng/ml,100 ng/ml or 1 000 ng/ml).The control group was not stimulated.After 24,48 and 72 h,cell proliferation was detected by MTT assay.The migration and invasion ability of SU-DHL-4 cells were detected by Transwell after 24 h of IP-10 stimulation.Flow cytometry was used to analyze the expression of P-glycoprotein (P-gp) and multidrug resistance-associated protein 1 (MRP1) after 48 h stimulation.Results:IP-10 could promote the proliferation of SU-DHL-4 cells at 10,100 and 1 000 ng/ml at 24 h and 48 h compared with control group (P＜0.05).At 24 h,the migration and invasion abilities were significantly increased in three IP-10 treatment groups (P＜0.05).At 48 h,there was no significant difference in P-gp and MRP1 expression between control group and three IP-10 treatment groups (P＞0.05).Conclusion:IP-10 can promote the proliferation of SU-DHL-4 cells and enhance their migration and invasion ability,but has no effect on the expression of drug resistance protein in SU-DHL-4 cells.

References:

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Memo

Memo:
陕西省卫生计生科研基金（编号：2016D053）
Last Update: 2020-01-14