|Table of Contents|

Effects of GNA on proliferation,apoptosis,migration and cell cycle distribution of human cervical cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 02
Page:
200-205
Research Field:
Publishing date:

Info

Title:
Effects of GNA on proliferation,apoptosis,migration and cell cycle distribution of human cervical cancer cells
Author(s):
Chen Jian
Department of Gynaecology and Obstetrics，Jinghua Hospital District,the First Affiliated Hospital of Henan University of Science and Technology，Henan Luoyang 471000,China.
Keywords:
gambogic acid; cervical cancer cell; cell apoptosis; cycle distribution
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2020.02.005
Abstract:
Objective:To study the effects of gambogic acid (GNA) on proliferation inhibition,apoptosis,migration and cell cycle distribution of human cervical cancer cells.Methods:Cervical cancer Hela was divided into blank control group,concentration of 25 μmol/L group,concentration of 50 μmol/L and 100 μmol/L concentration group,blank control group.MTT method and flow cytometry detected for 24 h,48 h and 72 h period,inhibition of proliferation and apoptosis.Transwell experiment detected cell migration.Flow cytometry detected the cell cycle distribution.The relative expression levels of Bcl-2,Bax,E-cadherin and NF-κB were detected by Western blot.Results:The inhibition rate of cervical cancer cells proliferation in the 25 μmol/L concentration group,50 μmol/L concentration group and 100 μmol/L concentration group were significantly higher than that in the blank control group at different time periods,and the inhibition rate of proliferation was increased with the increase of concentration and time (P＜0.05).The apoptosis rate of cervical cancer cells in the 25 μmol/L concentration group,50 μmol/L concentration group and 100 μmol/L concentration group was significantly higher than that of the blank control group at all time periods,and the apoptosis rate was increased with the increase of concentration and time (P＜0.05).The number of cell migration of cervical cancer cells in the 25 μmol/L concentration group,50 μmol/L concentration group and 100 μmol/L concentration group was decreased significantly compared with the control group,and the number of cell migration was decreased with the increase of concentration (P＜0.05).The higher the concentration of GNA was,the higher the proportion of cells in G0/G1 phase was and the lower the proportion of cells in G2/M and S phase was.Bcl-2 and NF-κB in the 25 μmol/L concentration group,50 μmol/L concentration group and 100 μmol/L concentration group were all lower than the blank control group (P＜0.05).Bax and E-cadherin expression were higher in the 25 μmol/L concentration group,50 μmol/L concentration group and 100 μmol/L concentration group than in the blank control group.Conclusion:GNA can promote apoptosis of cervical cancer cells,inhibit cell proliferation and migration,and its capacity is concentration-dependent.

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Last Update: 2019-11-29