|Table of Contents|

The effect and possible mechanism of non-SMC condensin I complex,subunit G (NCAPG) on thyroid cancer cell line B-CPAP cell proliferation and apoptosis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 02
Page:
206-209
Research Field:
Publishing date:

Info

Title:
The effect and possible mechanism of non-SMC condensin I complex,subunit G (NCAPG) on thyroid cancer cell line B-CPAP cell proliferation and apoptosis
Author(s):
Li Jian; Li Zhendong
Department of Head and Neck Surgery,Cancer Hospital of China Medical University,Liaoning Cancer Hospital & Insititute,Liaoning Shenyang 110042,China.
Keywords:
NCAPG; thyroid cancer; proliferation; apoptosis
PACS:
R736.1
DOI:
10.3969/j.issn.1672-4992.2020.02.006
Abstract:
Objective:To investigate the effects and possible mechanisms of NCAPG on thyroid cancer cell proliferation and apoptosis.Methods:The expression of NCAPG in B-CPAP cells was down-regulated by siRNA-NCAPG.The expression of NCAPG gene and protein were detected by qRT-PCR and Western blot respectively.To investigate the effects of NCAPG on cell proliferation,cells in each group was detected by CCK-8.To investigate the effect of NCAPG on apoptosis of B-CPAP cells,Annexin V/PI double staining was detected by flow cytometry,and the expressions of apoptosis-related proteins Caspase-3,Bax and Bcl-2 were detected by Western blot.Results:B-CPAP cells transfected with siRNA-NCAPG successfully reduced the level of NCAPG expression.The decreased expression of NCAPG inhibited the proliferation of B-CPAP cells.The results of flow cytometry showed that down-regulation of NCAPG expression could induce apoptosis of B-CPAP cells.Western blot showed that down-regulation of NCAPG expression promoted the expression of Caspase-3 and Bax in B-CPAP cells and inhibited the expression of Bcl-2.Conclusion:NCAPG induces the proliferation of B-CPAP cells,and inhibits its expression,which can promote cell apoptosis by regulating the mitochondrial pathway.

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Last Update: 2019-11-29