|Table of Contents|

Effects of lncRNA CASC9 on proliferation,apoptosis and 5-FU chemotherapy-resistant of gastric cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 02
Page:
195-200
Research Field:
Publishing date:

Info

Title:
Effects of lncRNA CASC9 on proliferation,apoptosis and 5-FU chemotherapy-resistant of gastric cancer cells
Author(s):
Bao Chenhui; Zhao Ying
Gastrointestinal Nutrition Surgery Department,Shengjing Affiliated Hospital of China Medical University,Liaonig Shenyang 110004,China.
Keywords:
gastric cancer; long non-coding RNA CASC9; P-gp; proliferation; apoptosis; chemotherapy-resistant
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2020.02.004
Abstract:
Objective:To research the role and mechanism of lncRNA CASC9 in proliferation,apoptosis and 5-FU chemotherapy-resistant of gastric cancer (GC) cells．Methods：The expression of CASC9 and P-gp in normal gastric mucosa cells (GSE-1) and GC cells (SGC-7901 and BGC-823) was detected by real-time fluorescent quantitative PCR.The expression of P-gp in SGC-7901 and BGC-823 cells after knockdown CASC9 was examined by RT-PCR.The changes in abilities of proliferation in GC cells after knockdown CASC9 and overexpressed P-gp were examined by CCK-8.Annexin V-APC staining was used to detect the apoptotic rates of each group at 48 h after transfection．Cell viability was detected after transfection and culture with 5-FU.Results：The expression levels of CASC9 and P-gp in GC cells were significantly increased as compared with those in normal gastric mucosa cells (P＜0.05 for both).Transfection with si-CASC9 down-regulated P-gp expression (P＜0.05).In GC cells,the abilities of proliferation and 5-FU chemotherapy-resistant were significantly reduced and apoptosis was increased after knockdown CASC9 (P＜0.05).And the overexpression of P-gp can reversed this phenomenon (P＜0.05).Conclusion：CASC9 can regulate the proliferation,apoptosis and 5-FU chemotherapy-resistant of GC cells by promoting the expression of P-gp,and adjust the occurrence and development of GC.

References:

［1］Siegel RL,Miller KD,Jemal A.Cancer statistics,2017［J］.CA:A Cancer Journal for Clinicians,2017,67(1):7-30.
［2］Chen W,Zheng R,Baade PD,et al.Cancer statistics in China,2015［J］.CA:A Cancer Journal for Clinicians,2016,66(2):115-132.
［3］Orditura M,Galizia G,Sforza V,et al.Treatment of gastric cancer［J］.World Journal of Gastroenterology,2014,20(7):1635-1649.
［4］Li X,Cai H,Zheng W,et al.An individualized prognostic signature for gastric cancer patients treated with 5-fluorouracil-based chemotherapy and distinct multi-omics characteristics of prognostic groups［J］.Oncotarget,2016,7(8):8743-8755.
［5］Cech TR,Steitz JA.The non-coding RNA revolution-trashing old rules to forge new ones［J］.Cell,2014,157(1):77-94.
［6］Schwartz S,Bernstein DA,Mumbach MR,et al.Transcriptome-wide mapping reveals widespread dynamic-regulated pseudouridylation of ncRNA and mRNA［J］.Cell,2014,159(1):148-162.
［7］Muers M.RNA:Genome-wide views of long non-coding RNAs［J］.Nature Reviews Genetics,2011,12(11):742.
［8］Ponting CP,Oliver PL,Reik W.Evolution and functions of long non-coding RNAs［J］.Cell,2009,136(4):629-641.
［9］Loewer S,Cabili MN,Guttman M,et al.Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells［J］.Nature Genetics,2010,42(12):1113-1117.
［10］Xu Q,Deng F,Qin Y,et al.Long non-coding RNA regulation of epithelial-mesenchymal transition in cancer metastasis［J］.Cell Death & Disease,2016,7(6):e2254.
［11］Yong W,Yu D,Jun Z,et al.Long non-coding RNA NEAT1,regulated by LIN28B,promotes cell proliferation and migration through sponging miR-506 in high-grade serous ovarian cancer［J］.Cell Death & Disease,2018,9(9):861.
［12］Zhu P,Wang Y,Huang G,et al.lnc-beta-Catm elicits EZH2-dependent beta-catenin stabilization and sustains liver CSC self-renewal［J］.Nature Structural & Molecular Biology,2016,23(7):631-639.
［13］Pan Z,Mao W,Bao Y,et al.The long non-coding RNA CASC9 regulates migration and invasion in esophageal cancer［J］.Cancer Medicine,2016,5(9):2442-2447.
［14］Liang Y,Chen X,Wu Y,et al.LncRNA CASC9 promotes esophageal squamous cell carcinoma metastasis through upregulating LAMC2 expression by interacting with the CREB-binding protein［J］.Cell Death and Differentiation,2018,25(11):1980-1995.
［15］Su X,Li G,Liu W.The long non-coding RNA cancer susceptibility candidate 9 promotes nasopharyngeal carcinogenesis via stabilizing HIF1alpha［J］.DNA and Cell Biology,2017,36(5):394-400.
［16］Jiang B,Li Y,Qu X,et al.Long non-coding RNA cancer susceptibility candidate 9 promotes doxorubicinresistant breast cancer by binding to enhancer of zeste homolog 2［J］.International Journal of Molecular Medicine,2018,42(5):2801-2810.
［17］Shang C,Sun L,Zhang J,et al.Silence of cancer susceptibility candidate 9 inhibits gastric cancer and reverses chemoresistance［J］.Oncotarget,2017,8(9):15393-15398.
［18］Hu X,Li Y,Kong D,et al.Long non-coding RNA CASC9 promotes LIN7A expression via miR-758-3p to facilitate the malignancy of ovarian cancer［J］.Journal of Cellular Physiology,2018(12):7.

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辽宁省科学技术计划项目（编号：201601122）
Last Update: 2019-11-29