|Table of Contents|

The combination of MEMO1 with IGF-IR in the regulation of invasion and metastasis in gastric cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 02
Page:
190-195
Research Field:
Publishing date:

Info

Title:
The combination of MEMO1 with IGF-IR in the regulation of invasion and metastasis in gastric cancer cells
Author(s):
Wang Zhi1; Li Heming1; Liang Shanshan2; Wang Ruoyu1
1.Department of Oncology,Affiliated Zhongshan Hospital of Dalian University,Liaoning Dalian 116001,China;2.The Key Laboratory of Biomarker High Throughput Screening and Target Translation of Breast and Gastrointestinal Tumor,Liaoning Dalian 116001,China.
Keywords:
MEMO1; IGF-IR; gastric cancer; EMT
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2020.02.003
Abstract:
Objective:To investigate the molecular mechanism and interaction of MEMO1 and IGF-IR in the regulation of invasion and metastasis in gastric cancer cell lines,and to explore a potential target for clinical treatment in the gastric cancer.Methods:Five different differentiated gastric cancer cell lines (MKN-7,NCI-N87,MGC-803,BGC-823,MKN-74) were selected.Transwell was used to compare migration abilities of five gastric cancer cell lines.Western blot was used to detect the different expression levels of MEMO1,IGF-IR and E-cad.The expressions of EMT markers were detected by Western blot analysis after knockdown of MEMO1 or IGF-IR gene instantaneously in one of MEMO1 & IGF-IR over-expression cell lines,MGC-803.IP assay was used to detect the interaction between MEMO1 and IGF-IR.TCGA database was used to analyze the relationship between mRNA expression level of MEMO1 and IGF-IR and long-term survival in gastric cancer patients.Results:IGF-IR was significantly positively correlated with the expression of MEMO1.The migration abilities of five gastric cancer cells were MKN-7

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Memo

Memo:
National Natural Science Foundation of China（No.81502124）;国家自然科学基金（编号：81502124）；2018年大连市科技创新基金计划（编号：2018J12SN063）；2019年大连市医学科学研究计划项目（编号：1911096）
Last Update: 2019-11-29