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The effect of ZIC1 over-expression on the proliferation of HCT-116 cells in colorectal cancer and the sensitivity of matrine alkaloids

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 03
Page:
386-389
Research Field:
Publishing date:

Info

Title:
The effect of ZIC1 over-expression on the proliferation of HCT-116 cells in colorectal cancer and the sensitivity of matrine alkaloids
Author(s):
Wang Jiefeng1Xiao Jintan1Sheng Zhenfang1Ding Houzhong2
1.Department of General Surgery,Kunshan Qiandeng People's Hospital,Jiangsu Kunshan 215300,China;2.Department of General Surgery,Kunshan Hospital Affiliated to Jiangsu University,Jiangsu Kunshan 215300,China.
Keywords:
colorectal cancerZIC1matrine alkaloids
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2019.03.006
Abstract:
Objective:To evaluate the effect of ZIC1 gene on the proliferation ability of cancer cells in colorectal cancer cells and changes of the sensitivity of matrine alkaloid drugs.Methods:Firstly,one cell line with lowest expression of ZIC1 was selected from the five kinds of colorectal cancer cell lines,and was infected by rLV-ZIC1-PGK-Puro or rLV-PGK-Puro followed by Western blot and qRT-PCR to assess ZIC1 protein/mRNA expression.Through CCK-8 and colony formation assay,the cell proliferation was detected.CCK-8 was also used to investigate changes of the sensitivity of matrine alkaloid.Results:The expression of ZIC1 mRNA/protein in HCT116 cell lines was significantly lower than that of the other four(P<0.001).The expression level of ZIC1 mRNA/protein in HCT116 cells with rLV-ZIC1-PGK-Puro was significantly higher than that in the control group(P<0.001).The results of CCK-8 showed that the proliferation of HCT116 cells was significantly reduced after ZIC1 gene overexpression(P<0.01).The clone formation rate of the experimental group was significantly lower than that in the control group(P=0.003).CCK-8 also found that HCT116 colorectal cancer cell with over-expressed ZIC1 had higher sensitivity of matrine and sophoridine.Conclusion:Over-expressed ZIC1 in HCT116 colorectal cancer cell can effectively inhibit the proliferation of cancer cells,and increase the sensitivity of matrine and sophoridine.

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Last Update: 2018-12-29