|Table of Contents|

Investigation the influence of lncRNA MEG3 on proliferation and autophagy in HCC cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 03
Page:
382-385
Research Field:
Publishing date:

Info

Title:
Investigation the influence of lncRNA MEG3 on proliferation and autophagy in HCC cells
Author(s):
Li Yan; Yang Daye; Zhang Dewei
Department of General Surgery，the Fourth Affiliated Hospital of China Medical University，Liaoning Shenyang 110032，China.
Keywords:
lncRNA; MEG3; autophagy; proliferation; HCC
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2019.03.005
Abstract:
Objective：To investigate the influence of lncRNA MEG3 on proliferation and autophagy in HCC cells.Methods：Real-time PCR method was used to detect the expression profile of MEG3 in 60 HCC samples and HCC cell lines.CCK-8 assay was performed to investigate the effect of poliferation in HCC cells.Using Western blot and immunofluorescence method to detect the effect of MEG3 on autophagy.Results：The expression of MEG3 was significantly downregulated in HCC tissues and cells(P<0.05).After overexpression of MEG3 in HCC cells，the proliferation of HCC cells was significantly decreased.And the expression of LC3-I to LC3-Ⅱ was reduced（P<0.05).Moreover，the immunofluorescence assay showed overexpression of MEG3 could decrease the LC3 fluorescence points.Conclusion：The expression of MEG3 in liver cancer tissue is lower than normal tissue.In vitro，MEG3 can inhibit the proliferation and autophagy of HCC cells.It is expected to be a potential target for the treatment of HCC.

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Memo

Memo:
辽宁省自然科学基金（编号：2014021094）
Last Update: 2018-12-29