|Table of Contents|

Preliminary study on expression and biological functions of miR-152 in acute myeloid leukemia patients bone marrow and cell lines

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 03
Page:
376-381
Research Field:
Publishing date:

Info

Title:
Preliminary study on expression and biological functions of miR-152 in acute myeloid leukemia patients bone marrow and cell lines
Author(s):
Liu Wenxia1; Wang Xiaoxue2; Li Yan2
1.Department of Hematology，the Central Hospital of Yingkou，Liaoning Yingkou 115000，China；2.Department of Hematology，the First Affiliated Hospital of China Medical University，Liaoning Shenyang 110001，China.
Keywords:
miR-152; acute myeloid leukemia; proliferation; apoptosis; cell cycles
PACS:
R733.7
DOI:
10.3969/j.issn.1672-4992.2019.03.004
Abstract:
Objective：To detect the expression level of miR-152 in acute myeloid leukemia patients bone marrow and cell lines.A preliminary study on the biological function of miR-152 in AML was also conducted.Methods：40 patients with AML and 20 cases with non-malignant blood disease were collected.AML cell lines U937，THP-1 and Kasumi-1 were cultured.Expression level of miR-152 was detected by RT-PCR.miR-152 mimics/inhibitor respectively transfected U937 cell lines and Kasumi-1 cell lines.U937 cell and Kasumi-1 cell proliferation were detected by CCK-8 method.Flow cytometry was used to detect cell apoptosis and cell cycle.Results：miR-152 was significantly decreased in AML patients bone marrow and cell lines compared with non-malignant hematologic disease control group.miR-152 mimics were transfected into U937 cell line.U937 cells were blocked in G0-G1 phase，and proliferation was inhibited and apoptosis was increased after transfection.miR-152 inhibitor were transfected into Kasumi-1 cell lines，showing that cells in G0-G1 were reduced，and proliferation was increased and apoptosis was decreased.Conclusion：Expression of miR-152 was significantly lower in AML patients bone marrow and cell lines than normal control.miR-152 plays a role of tumor suppressor genes in AML.Over expression of miR-152 can inhibit cell proliferation，promote apoptosis.

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