|Table of Contents|

miR-149-3p inhibits the proliferation,migration and invasion of bladder cancer cells by targeting S100A4

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 01
Page:
12-17
Research Field:
Publishing date:

Info

Title:
miR-149-3p inhibits the proliferation,migration and invasion of bladder cancer cells by targeting S100A4
Author(s):
Xie ShunmingWang WenxiaoZhao Wenwu
Department of Urology Surgery,Deyang People's Hospital,Sichuan Deyang 618000,China.
Keywords:
bladder cancermiR-149-3pS100A4invasion behavior
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2019.01.004
Abstract:
Objective:To investigate the inhibitory effect of miR-149-3p on the proliferation,migration and invasion of bladder cancer by targeting S100A4 and its mechanism.Methods:qPCR was used to detect the overexpression of miR-149-3p.The proliferation of bladder cancer cells was detected by CCK-8 cell proliferation assay.Scratch healing assay and Transwell invasion assay were used to detect the effect of miR-149-3p on the migration and invasion ability of bladder cancer cells.Dual luciferase reporter gene was used to detect miR-149-3p and S100A4 interaction.Transwell invasion assay examined the ectopic expression of S100A4 reversing the antitumor effect of miR-149-3p.Results:Overexpression of miR-149-3p significantly inhibited the growth of cultured bladder cancer cells for 48 hours.The miR-149-3p overexpression showed anti-migration and invasion in bladder cancer cells.miR-149-3p can specifically bind to the 3' UTR of S100A4 and regulate the expression of S100A4.Overexpression of S100A4 can reverse the inhibition of cell migration and invasion capacity caused by miR-149-3p.Conclusion:miR-149-3p plays an important role in the development and progression of bladder cancer.It may affect the proliferation and migration of bladder cancer cells by regulating the expression of S100A4.

References:

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Last Update: 2018-11-30