|Table of Contents|

Effect of sodium selenite on invasion and migration of lung cancer stem cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 01
Page:
17-21
Research Field:
Publishing date:

Info

Title:
Effect of sodium selenite on invasion and migration of lung cancer stem cells
Author(s):
Zhang YaoZhong ZhaokunZhang EnningLiu LeiChen WeijunLuo XiaodongQu Weiqing
Department of Oncology,Yantaishan Hospital of Yantai,Shandong Yantai 264000,China.
Keywords:
lung cancer stem cellssodium seleniteproliferationinvasionmigration
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2019.01.005
Abstract:
Objective:To explore the effect of sodium selenite on the invasion and migration of the lung cancer stem cells.Methods:Magnetic bead separation was used to purify lung cancer stem cells.The effect of sodium selenite on the cell activity of the lung cancer stem cells was measured by CKK-8 assay.After the lung cancer stem cells were treated with sodium selenite,the ability of cell invasion was detected by Transwell invasion assay.Wound healing cell migration assay was used to analyze the ability of cell migration of lung cancer stem cells.The protein levels of SBP1 and Ki67 were detected using Western blot.Results:The CD133+lung cancer stem cells were successfully enriched to a content of 82%.Sodium selenite significantly inhibited the cell activity of the lung cancer stem cells in a dose-dependent manner.Sodium selenite also inhibited the cell invasion and migration of the lung cancer stem cells.In addition,after the stem cells were treated with sodium selenite,the protein expression level of SBP1 was up-regulated and Ki67 level was decreased.Compared with control group,sodium selenite significantly inhibited the growth of lung cancer by A549 transplantation.Conclusion:Sodium selenite inhibits the cell activity,invasion and migration of the lung cancer stem cells,indicating that sodium selenite is a potential drug for treatment of lung cancer.

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Memo

Memo:
National Natural Science Foundation of China(No.81272588);国家自然科学基金(编号:81272588)
Last Update: 2018-11-30