|Table of Contents|

Overexpression of miR-139 reduces TRAIL tolerance in acute myeloid leukemia

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 01
Page:
9-12
Research Field:
Publishing date:

Info

Title:
Overexpression of miR-139 reduces TRAIL tolerance in acute myeloid leukemia
Author(s):
Yao Jinxiao1; Yang Ruyu1; Ma Hailong1; Li Chao1; Wei Xudong2
1.Department of Hematology，Nanyang City Center Hospital Affiliated to Zhengzhou University，Henan Nanyang 473000，China；2.Department of Hematology，Cancer Hospital Affiliated to Zhengzhou University，Henan Zhengzhou 450008，China.
Keywords:
miR-139; acute myeloid leukemia; TRAIL tolerance; apoptosis
PACS:
R733.71
DOI:
10.3969/j.issn.1672-4992.2019.01.003
Abstract:
Objective：To investigate the effect of overexpression of miR-139 on TNF-related apoptosis inducing ligand (TRAIL) tolerance in acute myeloid leukemia.Methods：K562，HL-60 cells and corresponding multidrug resistant K562/A02 and HL-60/ADM cells in human acute myeloid leukemia were treated with concentration of 100，200，300 and 400 ng/ml TRAIL.IC50 value was calculated by CCK8 method.The sensitivity of tolerance in acute myeloid leukemia cells to TRAIL was evaluated by IC50 method.The expression of miR-139 in TRAIL sensitive cells and drug-resistant cells was detected by RT-PCR.miR-139 mimics was transfected into TRAIL resistant strain by liposome 2000.The transfection effect was tested by RT-PCR.Cell viability and IC50 value were detected by CCK8.The rate of apoptosis was detected by flow cytometry.Results：In K562，K562/A02，HL-60 and HL-60/ADM cells，HL-60/ADM cells were the most sensitive，while K562 cells had the strongest resistance to TRAIL.Compared with the sensitive cell line HL-60/ADM，miR-139 expression in the drug resistant cell line K562 was decreased significantly.After transfection of miR-139 mimics，the expression of miR-139 and apoptosis rate in K562 cells were increased significantly，while cell viability and IC50 were decreased significantly.Conclusion：miR-139 is low in TRAIL resistant cell line K562.Overexpression of miR-139 may reduce the tolerance of K562 cells to TRAIL by promoting TRAIL induced apoptosis.

References:

［1］Dhner H，Weisdorf DJ，Bloomfield CD.Acute myeloid leukemia［J］.N Engl J Med，2015，373(12)：1136-1152.
[2]Maurolizcano M，Lópezrivas A.Glutamine metabolism regulates FLIP expression and sensitivity to TRAIL in triple-negative breast cancer cells［J］.Cell Death & Disease，2018，9(2)：205-218.
[3]Tang G.Trichosanthin enhances sensitivity of non-small cell lung cancer (NSCLC) TRAIL-resistance cells［J］.Int J Biol Sci，2018，14(2)：217-227.
[4]Hu W，Jia X，Gao Y，et al.Chaetospirolactone reverses the apoptotic resistance towards TRAIL in pancreatic cancer［J］.Biochem Biophys Res Commun，2018，495(1)：621-628.
[5]Yang T，Lan J，Huang Q，et al.Embelin sensitizes acute myeloid leukemia cells to TRAIL through XIAP inhibition and NF-κB inactivation［J］.Cell Biochem Biophy，2015，71(1)：291-297.
[6]Liu J，Li C，Jiang Y，et al.Tumor-suppressor role of miR-139-5p in endometrial cancer［J］.Cancer Cell Inte，2018，18(1)：51-59.
[7]Xu K，Shen K，Liang X，et al.MiR-139-5p reverses CD44+/CD133+-associated multidrug resistance by downregulating NOTCH1 in colorectal carcinoma cells［J］.Oncotarget，2016，7(46)：75118-75129.
[8]Krowiorz K，Ruschmann J，Lai C，et al.MiR-139-5p is a potent tumor suppressor in adult acute myeloid leukemia［J］.Blood Cancer J，2016，6(12)：e508-e511.
[9]Sun Y，Lu F，Han X，et al.MiR-424 and miR-27a increase TRAIL sensitivity of acute myeloid leukemia by targeting PLAG1［J］.Oncotarget，2016，7(18)：25276-25290.
[10]Zhou J，Lu X，Tan TZ，et al.X-linked inhibitor of apoptosis inhibition sensitizes acute myeloid leukemia cell response to TRAIL and chemotherapy through potentiated induction of proapoptotic machinery［J］.Mol Oncol，2018，12(1)：33-47.
[11]Fadeev RS，Solovieva ME，Slyadovskiy DA，et al.Inhibition of NF-κB activation decreases resistance in acute myeloid leukemia cells to TRAIL-induced apoptosis in multicellular aggregates［J］.Biofizika，2015，60(6)：1146-1150.
[12]Tyagi N，Arora S，Deshmukh SK，et al.Exploiting nanotechnology for the development of microrna-based cancer therapeutics［J］.J Biomed Nanotechnol，2016，12(1)：28-42.
[13]Lee WL，Wang J，Wah LO，et al.MicroRNA and heart failure［J］.Inte J Mol Sci，2016，17(4)：502-532.
[14]Bourguignon LY.Matrix hyaluronan promotes specific microrna upregulation leading to drug resistance and tumor progression［J］.Inte J Mol Sci，2016，17(4)：517-528.
[15]Gu W，Li X，Wang J.miR-139 regulates the proliferation and invasion of hepatocellular carcinoma through the WNT/TCF-4 pathway［J］.Oncol Rep，2014，31(1)：397-404.
[16]Yu X，Ma C，Fu L，et al.MicroRNA-139 inhibits the proliferation，migration and invasion of gastric cancer cells by directly targeting ρ-associated protein kinase 1［J］.Oncol Lett，2018，15(4)：5977-5982.
[17]Ren Y，Zhu H，Chi C，et al.MiRNA-139 regulates oral cancer Tca8113 cells apoptosis through Akt signaling pathway［J］.Cancer，2015，8(5)：4588-4594.
[18]Zhang HD，Sun DW，Mao L，et al.MiR-139-5p inhibits the biological function of breast cancer cells by targeting Notch1 and mediates chemosensitivity to docetaxel［J］.Biochem Biophy Res Commun，2015，465(4)：702-713.
[19]Liu H，Yin Y，Hu Y，et al.miR-139-5p sensitizes colorectal cancer cells to 5-fluorouracil by targeting NOTCH-1［J］.Pathol-Res Pract，2016，212(7)：643-649.
[20]Emmrich S，Engeland F，El-Khatib M，et al.miR-139-5p controls translation in myeloid leukemia through EIF4G2［J］.Oncogene，2016，35(14)：1822-1831.
[21]Engeland F，Emmrich S，Kuipers J，et al.Investigating the role of miR-139 and miR-582 in AML1-ETO associated leukemia［J］.Klinische Pdiatrie，2012，224(3)：457-474.

Memo

Memo:
National Natural Science Foundation of China(No.81170520）；国家自然科学基金面上项目（编号：81170520）
Last Update: 2018-11-30