|Table of Contents|

The molecular mechanism of Ghrelin induces breast cancer cell MDA-MB-231 proliferation

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 10
Page:
1488-1491
Research Field:
Publishing date:

Info

Title:
The molecular mechanism of Ghrelin induces breast cancer cell MDA-MB-231 proliferation
Author(s):
Zhu Jianhua; Jia Min; Wang Yueqin; Huang Yan
Laboratory of Clinical Immunology,Wuhan No.1 Hospital,Tongji Medical College,Huazhong University of Science and Technology(HUST),Hubei Wuhan 430022,China.
Keywords:
MDA-MB-231; Ghrelin; proliferation; GHSR; mTOR
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2018.10.002
Abstract:
Objective:To investigate the molecular mechanism of Ghrelin induces breast cancer cell MDA-MB-231 proliferation.Methods:Human breast cancer cell MDA-MB-231 were treated with Ghrelin,［D-Lys3］-GHRP-6 and Rapamycin.The proliferation of MDA-MB-231 was determined by MTT assay and BrdU assay.The expression of GHSR and phosphorylation of mTOR,p70S6K,S6 was detected by Western blot.Results:The proliferation of MDA-MB-231 cells was increased by Ghrelin.Western blot showed that GHSR was expressed on MDA-MB-231 cells and Ghrelin induced rapid phosphorylation of mTOR,p70S6K and S6,which was lower in D-L-GHRP group compared with the Ghrelin group.Conclusion:The results demonstrate that Ghrelin induces MDA-MB-231 cells proliferation through binding to GHSR and activating mTOR/p70S6K/S6 signaling pathway.

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Memo

Memo:
National Natural Science Foundation of China（No.81502222）；国家自然科学基金（编号：81502222）;湖北省自然科学基金（编号：2013CFB370）；武汉市卫计委课题（编号：武卫2007-43）
Last Update: 2018-04-02