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The molecular mechanism of Ghrelin induces breast cancer cell MDA-MB-231 proliferation

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

2018 10
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The molecular mechanism of Ghrelin induces breast cancer cell MDA-MB-231 proliferation
Zhu JianhuaJia MinWang YueqinHuang Yan
Laboratory of Clinical Immunology,Wuhan No.1 Hospital,Tongji Medical College,Huazhong University of Science and Technology(HUST),Hubei Wuhan 430022,China.
Objective:To investigate the molecular mechanism of Ghrelin induces breast cancer cell MDA-MB-231 proliferation.Methods:Human breast cancer cell MDA-MB-231 were treated with Ghrelin,[D-Lys3]-GHRP-6 and Rapamycin.The proliferation of MDA-MB-231 was determined by MTT assay and BrdU assay.The expression of GHSR and phosphorylation of mTOR,p70S6K,S6 was detected by Western blot.Results:The proliferation of MDA-MB-231 cells was increased by Ghrelin.Western blot showed that GHSR was expressed on MDA-MB-231 cells and Ghrelin induced rapid phosphorylation of mTOR,p70S6K and S6,which was lower in D-L-GHRP group compared with the Ghrelin group.Conclusion:The results demonstrate that Ghrelin induces MDA-MB-231 cells proliferation through binding to GHSR and activating mTOR/p70S6K/S6 signaling pathway.


[1] Chen X,Duan N,Zhang C,et al.Surviving and tumorigenesis:Molecular mechanisms and therapeutic strategies [J].J Cancer,2016,7(3):314-323.
[2] Lin TC,Liu YP,Chan YC,et al.Ghrelin promotes renal cell carcinoma metastasis via snail activation and is associated with poor prognosis [J].Pathol,2015,237:50-61.
[3] Lin TC,Hsiao M.Ghrelin and cancer progression [J].Biochim Biophys Acta,2017,1868(1):51-57.
[4] Waseem T,Javaid-Ur-Rehman,Ahmad F,et al.Role of Ghrelin axis in colorectal cancer:A novel association [J].Peptides,2008,29:1369-1376.
[5] Yeh AH,Jeffery PL,Duncan RP,et al.Ghrelin and a novel preproGhrelin isoform are highly expressed in prostate cancer and Ghrelin activates mitogen-activated protein kinase in prostate cancer [J].Clin Cancer Res,2005,11:8295-8303.
[6] Kraus D,Reckenbeil J,Wenghoefer M,et al.Ghrelin promotes oral tumor cell proliferation by modifying GLUT1 expression [J].Cell Mol Life Sci,2016,73:1287-1299.
[7] Waseem T,Duxbury M,Ashley SW,et al.Ghrelin promotes intestinal epithelial cell proliferation through PI3K/Akt pathway and EGFR trans-activation both converging to ERK1/2 phosphorylation [J].Peptides,2014,52:113-121.
[8] Chung H,Li E,Kim Y,et al.Multiple signaling pathways mediate Ghrelin-induced proliferation of hippocampal neural stem cells [J].Endocrinol,2013,218:49-59.
[9] Grnberg M,Ahlin C,Naeser Y,et al.Ghrelin is a prognostic marker and a potential therapeutic target in breast cancer [J].PLoS One,2017,12(4):e0176059.
[10] Grnberg M,Fjallskog ML,Jirstrom K,et al.Expression of Ghrelin is correlated to a favorable outcome in invasive breast cancer [J].Acta Oncol,2012,51(3):386-393.
[11] Schally AV,Varga JL.Antagonistic analogs of growth hormone-releasing hormone:New potential antitumor agents [J].Trends Endocrinol Metab,1999,10(10):383-391.
[12] Jeffery PL,Murray RE,Yeh AH,et al.Expression and function of the Ghrelin axis,including a novel preproGhrelin isoform,in human breast cancer tissues and cell lines [J].Endocr Relat Cancer,2005,12(4):839-850.
[13] D Nikolopoulos,S Theocharis,G Kouraklis.Ghrelin:A potential therapeutic target for cancer [J].Regul Pept,2010,163:7-17.
[14] Yu H,Xu G,Fan X,et al.The effect of Ghrelin on cell proliferation in small intestinal IEC-6 cells [J].Biomed Pharmacother,2013,67:235-239.
[15] Lien GS,Lin CH,Yang YL,et al.Ghrelin induces colon cancer cell proliferation through the GHS-R,Ras,PI3K,Akt,and mTOR signaling pathways [J].Eur J Pharmacol,2016,5(776):124-131.


National Natural Science Foundation of China(No.81502222);国家自然科学基金(编号:81502222);湖北省自然科学基金(编号:2013CFB370);武汉市卫计委课题(编号:武卫2007-43)
Last Update: 2018-04-02