|Table of Contents|

Effect of miR-124 on the radiosensitivity of cervical cancer Hela cells and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 06
Page:
818-821
Research Field:
Publishing date:

Info

Title:
Effect of miR-124 on the radiosensitivity of cervical cancer Hela cells and its mechanism
Author(s):
Wang Mengjie1; 2; Meng Bi1; 2; Gao Fei2; Li Hao2; Ye Ting2; Liu Yangchen2
1.Graduate School of Bengbu Medical College，Anhui Bengbu 233003,China；2.Taixing People's Hospital，Jiangsu Taixing 225400,China.
Keywords:
miR-124; cervical cancer; radiosensitivity
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2018.06.002
Abstract:
Objective:To investigate the effect of miR-124 on radiosensitivity of cervical cancer Hela cells and its mechanism.Methods:The miR-124 mimics and miR-124 inhibitor were transfected into Hela cells.Cell cycle experiment showed that miR-124 could influence cell cycle.The important role of miR-124 in radiation sensitivity of Hela cells was confirmed by colony-formation assay and cell apoptosis assay.The target gene of miR-124 was confirmed by luciferase reporter assay,RT-PCR and Western blot.Results:The percentage of G0/G1 phase cells in miR-124 mimics group was higher than that in the control group，and the percentage of cells in S phase was lower than that of the control group(all P＜0.05).Overexpression of miR-124 could inhibit Hela cells ability of colony formation，and SER was 1.49.Flow cytometric analysis showed that the overexpression of miR-124 had an effect on the proliferation and apoptosis of cervical cancer cells after irradiation（P＜0.05).Inhibition of miR-124 would lead to the contrary results（P＜0.05).Luciferase reporter gene test,RT-PCR and Western blot experiments showed that STAT3 was a direct target gene of miR-124（all P＜0.05）.Conclusion:miR-124 may contribute to enhancing radiosensitivity of cervical cancer Hela cells by targeting STAT3.

References:

［1］ Chen W,Zheng R,Baade PD,et al.Cancer statistics in China,2015［J］.CA Cancer J Clin,2016,66(2):115-132.
［2］ Vordermark D.Radiotherapy of cervical cancer［J］.Oncol Res Treat,2016,39(9):516-520.
［3］ Schaue D,McBride WH.Opportunities and challenges of radiotherapy for treating cancer［J］.Nat Rev Clin Oncol,2015,12(9):527-540.
［4］ Wang Mengjie,Liu Yangchen.Research progress on the sensitivity of small RNA and cervical cancer radiotherapy［J］.Oncology Progress,2016,14(9):843-846.［王梦洁,刘阳晨.微小RNA与宫颈癌放疗敏感性的研究进展［J］.癌症进展,2016,14(9):843-846.］
［5］ Zhao Y,Ling Z,Hao Y,et al.MiR-124 acts as a tumor suppressor by inhibiting the expression of sphingosine kinase 1 and its downstream signaling in head and neck squamous cell carcinoma［J］.Oncotarget,2017,8(15):25005-25020.
［6］ El Bezawy R,Cominetti D,Fenderico N,et al.miR-875-5p counteracts epithelial-to-mesenchymal transition and enhances radiation response in prostate cancer through repression of the EGFR-ZEB1 axis［J］.Cancer Lett,2017,395:53-62.
［7］ Huang D,Bian G,Pan Y,et al.MiR-20a-5p promotes radio-resistance by targeting Rab27B in nasopharyngeal cancer cells［J］.Cancer Cell Int,2017,17(32):1-11.
［8］ Zheng R,Liu Y,Zhang X,et al.miRNA-200c enhances radiosensitivity of esophageal cancer by cell cycle arrest and targeting p21［J］.Biomed Pharmacother,2017,90:517-523.
［9］ Sun Y,Guo BF,Xu LB,et al.Stat3-siRNA inhibits the growth of gastric cancer in vitro and in vivo［J］.Cell Biochem Funct,2015,33(7):495-502.
［10］ Kunigal S,Lakka SS,Sodadasu PK,et al.Stat3-siRNA induces Fas-mediated apoptosis in vitro and in vivo in breast cancer［J］.Int J Oncol,2009,34(5):1209-1220.
［11］ Chen YJ,You ML,Chong QY,et al.Autocrine human growth hormone promotes invasive and cancer stem cell-like behavior of hepatocellular carcinoma cells by STAT3 dependent inhibition of CLAUDIN-1 expression［J］.Int J Mol Sci,2017,18(6):1-21.
［12］ Li X,Wang H,Lu X,et al.Silencing STAT3 with short hairpin RNA enhances radiosensitivity of human laryngeal squamous cell carcinoma xenografts in vivo［J］.Exp Ther Med,2010,1(6):947-953.
［13］ Wang Hairu,Li Xiaoming,Li Chunlin,et al.Enhancing radiosensitivity of RNA interference targeting silence STAT3 gene on human laryngeal carcinoma cells［J］.Chinese Archives of Otolaryngology-Head and Neck Surgery,2009,16(11):623-627.［王海茹,李晓明,李春林,等.RNA干扰沉默STAT3基因增强人喉癌细胞放射敏感性的实验［J］.中国耳鼻咽喉头颈外科,2009,16(11):623-627.］
［14］ Zhang C,Yang X,Zhang Q,et al.STAT3 inhibitor NSC74859 radiosensitizes esophageal cancer via the downregulation of HIF-1alpha［J］.Tumour Biol,2014,35(10):9793-9799.

Memo

Memo:
蚌埠医学院研究生科研创新计划(编号：Byycx1624)
Last Update: 2018-01-29