|Table of Contents|

Tumor associated neutrophils promote the migration of human osteosarcoma cells SOSP-9607 and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 06
Page:
813-817
Research Field:
Publishing date:

Info

Title:
Tumor associated neutrophils promote the migration of human osteosarcoma cells SOSP-9607 and its mechanism
Author(s):
Yang TongleiLiu TaoMa QiongQiu XiuchunLiu YunyanWen YanhuaMa Baoan
Department of Orthopedic Oncology Institute of PLA,Tangdu Hospital,The Fourth Military Medical University,Shaanxi Xi'an 710038,China.
Keywords:
osteosarcomatumor-associated neutrophilscytokines
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2018.06.001
Abstract:
Objective:To investigate the effect of tumor associated neutrophils on the migration of osteosarcoma cells and its possible mechanism.Methods:The extent of neutrophil infiltration in osteosarcoma specimens was studied by HE and immunohistochemical staining.Neutrophils were cultured with human osteosarcoma cells SOSP-9607,and the migration of SOSP-9607 cells co-cultured with different proportions of neutrophils were monitored using xCELLigence RTCA DP.Cytokine microarray was performed to detect the changes of different cytokines after co-culture.Results:HE and immunohistochemical staining showed that there was infiltration of neutrophils in clinical osteosarcoma specimens.xCELLigence RTCA DP showed that the migration ability of SOSP-9607 cells was significantly enhanced when they were co-cultured with neutrophils.Cytokine microarray analysis showed that IL-6,IL-1β,Angiogenin,IGFBP-1 and other cytokines were significantly increased.Conclusion:Neutrophils can promote SOSP-9607 migration when co-cultured with SOSP-9607 cells,which is likely to be achieved via a variety of cytokines such as IL-6,IL-1β,Angiogenin and IGFBP-1.

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Memo

Memo:
National Natural Science Foundation of China(No.81372297);国家自然科学基金(编号:81372297)
Last Update: 2018-01-29