|Table of Contents|

The anti-lung cancer mechanisms of Silymarin via inhibiting myeloid-derived suppressor cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 06
Page:
822-827
Research Field:
Publishing date:

Info

Title:
The anti-lung cancer mechanisms of Silymarin via inhibiting myeloid-derived suppressor cells
Author(s):
Wu Tiancong1; Wang Xia2; Zhu Xixu1
1.Department of Radiation Oncology,Nanjing General Hospital of Nanjing Command,PLA,Jiangsu Nanjing 210002,China;2.Department of Radiation Oncology,The Affiliated Jiangning Hospital of Nanjing Medical University,Jiangsu Nanjing 211100,China.
Keywords:
Silymarin; lung cancer; MDSC; CD8+ T cell
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2018.06.003
Abstract:
Objective:To study the anti-lung cancer mechanisms of Silymarin via inhibiting myeloid-derived suppressor cells（MDSC）.Methods:We developed a mouse xenograft model of Lewis lung cancer cells(LLC) in C57/BL6 mice and administered(i.g) Silymarin(25,50 mg/kg,once a day) dose dependently.Tumor volumes and mice body weight were measured.The expression of proliferating cell nuclear antigen(PCNA) related to tumor proliferation was detected by immunohistochemistry(IHC) staining.The apoptosis of LLC cells was detected by TUNEL staining.Tumor-infiltrating CD8+ T cells in LLC xenografts were detected by IHC staining.The percentage of CD11b+Gr-1+ MDSC was detected by flow cytometry.RNA was extracted from tumor tissue of mice and the expressions of Arg-1,iNOS2 and MMP9 mRNA were determined by Q-PCR.Results:Silymarin significantly suppressed tumor growth and induced apoptosis of cells in tumor tissues at a dose of 25 and 50 mg/kg.Silymarin treatment enhanced the infiltration and function of CD8+ T cells.Finally,Silymarin reduced the proportion of myeloid-derived suppressor cells(MDSC) in the tumor tissue and the mRNA expressions of inducible nitric oxide synthases-2(iNOS2),arginase-1(Arg-1) and MMP9,which indicated that the function of MDSC in tumor tissues was suppressed.Conclusion:Altogether,our data here showed that Silymarin inhibited the MDSC and promoted the infiltration and function of CD8+ T cells thus suppressed the growth of LLC xenografts,which provides evidence for the possible use of Silymarin against lung cancer.

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Last Update: 2018-01-29