|Table of Contents|

Effects of miR-300 on the proliferation,apoptosis and cell cycle of ovarian cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 06
Page:
868-871
Research Field:
Publishing date:

Info

Title:
Effects of miR-300 on the proliferation,apoptosis and cell cycle of ovarian cancer cells
Author(s):
Yuan Yuan1; Chang Xiaobin2; Yao Anmei1; Wang Jing1
1.Department of Gynecological Oncology;2.Department of Radiotherapy，Shaanxi Provincial Tumor Hospital,Shaanxi Xi'an 710061,China.
Keywords:
miR-300; ovarian cancer; proliferation; apoptosis; cell cycle
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2017.06.008
Abstract:
Objective:To investigate the effects of miR-300 on the proliferation,apoptosis,and cell cycle of humanovarian cancer cells.Methods：The expression of miR-300 in the normal ovarian epithelial cell and ovarian cancer cells,and the silencing efficiency of miR-300 siRNA was detected by quantitative Real time PCR(qRT-PCR).After siRNA and NC transfected into ovarian cancer cells for 48 hours，the proliferation of ovarian cancer cells were analyzed by CCK-8,the apoptosis and cell cycle of ovarian cancer cells was analyzed by flow cytometry.Results：The expression of miR-300 was significantly upregulated in ovarian cancer cells comparing with normal ovarian epithelial cells and could be effectively silenced by miR-300 siRNA.Proliferation of ovarian cancer cells were inhibited after transfected miR-300 siRNA,but cells apoptosis were promoted.Cell cycle of ovarian cancer cells were arrested at stage of G1 after transfected miR-300 siRNA.Conclusion：miR-300 play an important role on the proliferation,apoptosis and cell cycle of ovarian cancer cells.

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Memo

Memo:
陕西省自然科学基础研究计划项目（编号：S2013JC10400）
Last Update: 2017-01-26