|Table of Contents|

The effect of TSPAN8 on cell proliferation of human lung cancer A549 cells and the mechanisms

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 06
Page:
864-867
Research Field:
Publishing date:

Info

Title:
The effect of TSPAN8 on cell proliferation of human lung cancer A549 cells and the mechanisms
Author(s):
Gao Hui1; Yin Yujing2; Bayaer1; Lv Yihua1
1.Department of Cancer Integrative Internal Medicine;2.Department of Pathology,Baotou Cancer Hospital,Inner Mongolia Baotou 014030,China.
Keywords:
TSPAN8; lung cancer; proliferation
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2017.06.007
Abstract:
Objective:To investigate the effect and mechannisms of TSPAN8 gene on cell proliferation of A549.Methods:Human lung cancer cells A549 was transfected with pCMV-TSPAN8 plasmid.The proliferation of A549 cells was determined by MTT assay,flow cytometry was applied to detect the impact of TSPAN8 over-expression on cell cycle.The effects of TSPAN8 gene on expression on Rb1,E2F1,C-myc,CDK4 and Cyclin D1,and the phosphorylation of ERK in the A549 cells were determined using Real-time PCR and Western blot assay.Results:TSPAN8 gene could promote cell proliferation of human lung cancer cells in vitro.Western blot and Real-time PCR showed that protein and mRNA expression of E2F1,C-myc,CDK4 and Cyclin D1 were up-regulated after transfected with TSPAN8 gene in the A549 cells,but expression of Rb1 was down-regulated.At the same time,the phosphorylation of ERK was also activated as well.Conclusion:TSPAN8 gene could promote cell proliferation and metastasis of human lung cancer cells,and its effect might through facilitation of expression of Rb1,E2F1,C-myc,CDK4 and Cyclin D1,and activation of the ERK1/2 signal pathway.

References:

[1]Gadgeel SM.Personalized therapy of non-small cell lung cancer(NSCLC)［J］.Adv Exp Med Biol,2016,890:203-222.
[2]Amendola BE,Amendola MA,Perez N,et al.Local failure after primary radiotherapy in lung cancer:Is there a role for SBRT［J］?Rep Pract Oncol Radiother,2015,20(6):440-445.
[3]Alharbi KK,Ali Khan I,Syed R,et al.Association of JAZF1 and TSPAN8/LGR5 variants in relation to type 2 diabetes mellitus in a Saudi population［J］.Diabetol Metab Syndr,2015,7:92.
[4]Nazarenko I,Rana S,Baumann A,et al.Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation［J］.Cancer Res,2010,70(4):1668-1678.
[5]Yue S,Mu W,Zoller M.TSPAN8 and CD151 promote metastasis by distinct mechanisms［J］.Eur J Cancer,2013,49(13):2934-2948.
[6]Wei L,Li Y,Suo Z.TSPAN8 promotes gastric cancer growth and metastasis via ERK MAPK pathway［J］.Int J Clin Exp Med,2015,8(6):8599-8607.
[7]Berthier-Vergnes O,Kharbili ME,De la Fouchardière A,et al.Gene expression profiles of human melanoma cells with different invasive potential reveal TSPAN8 as a novel mediator of invasion［J］.Br J Cancer,2011,104(1):155-165.
[8]Pan SJ,Wu YB,Cai S,et al.Over-expression of tetraspanin 8 in malignant glioma regulates tumor cell progression［J］.Biochem Biophys Res Commun,2015,458(3):476-482.
[9]Roberts E,Cossigny DA,Quan GM.The role of vascular endothelial growth factor in metastatic prostate cancer to the skeleton［J］.Prostate Cancer,2013,2013:418340.
[10]Anami K,Oue N,Noguchi T,et al.TSPAN8,identified by Escherichia coli ampicillin secretion trap,is associated with cell growth and invasion in gastric cancer［J］.Gastric Cancer,2016,19(2):370-380.
[11]Fang TT,Sun XJ,Chen J,et al.Long non-coding RNAs are differentially expressed in hepatocellular carcinoma cell lines with differing metastatic potential［J］.Asian Pac J Cancer Prev,2014,15(23):10513-10524.
[12]Greco C,Bralet MP,Ailane N,et al.E-cadherin/p120-catenin and tetraspanin Co-029 cooperate for cell motility control in human colon carcinoma［J］.Cancer Res,2010,70(19):7674-7683.
[13]Ahn SM,Jang SJ,Shim JH,et al.Genomic portrait of resectable hepatocellular carcinomas:Implications of RB1 and FGF19 aberrations for patient stratification［J］.Hepatology,2014,60(6):1972-1982.
[14]Dumanskiy YV,Stoliarova OY,Syniachenko OV,et al.Endothelial dysfunction of vessels at lung cancer［J］.Exp Oncol,2015,37(4):277-280.
[15]Li X,Yang L,Shuai T,et al.MiR-433 inhibits retinoblastoma malignancy by suppressing Notch1 and PAX6 expression［J］.Biomed Pharmacother,2016,82:247-255.
[16]Liao X,Zhou X,Mak NK,et al.Tryptanthrin inhibits angiogenesis by targeting the VEGFR2-mediated ERK1/2 signalling pathway［J］.PLoS One,2013,8(12):e82294.

Memo

Memo:
-
Last Update: 2017-01-26