|Table of Contents|

The influence of Ca2+ in prostate cancer cells LNCaP by DHT and FLU

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 06
Page:
859-863
Research Field:
Publishing date:

Info

Title:
The influence of Ca2+ in prostate cancer cells LNCaP by DHT and FLU
Author(s):
Xie Jiaokui1Song Yin'e1Chen Haiwen2Zhang Shengru1Tian Hong3
1.Department of Urology,Ankang Central Hospital,Shaanxi Ankang 725000,China;2.Department of Urology,the Second Affiliatted Hospital of Medical School of Xi'an Jiaotong University,Shaanxi Xi'an 710004,China;3.The Research Center of Reproductive Medicine of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China.
Keywords:
dihydrotestosterone(DHT)fluorine(FLU)LNCaPcalciumion
PACS:
R737.25
DOI:
10.3969/j.issn.1672-4992.2017.06.006
Abstract:
Objective:To investigate the change of Ca2+ and discuss the mechanism of Ca2+ changes in LNCaP cells by DHT and FLU.Methods:To abtain drogen-dependent(LNCaP) prostate cancer cell lines,by cell culture,to abtain stable cell strains.The androgen receptor agonist DHT and the androgen receptor antagonist FLU acted on the culture prostate cancer cell line of androgen at different concentrations drugs.We extracted cells at different time points of LNCaP under the interventions.By fluorescent dye load measured,to compare the Ca2+ differences at different time points and under the action of different drug concentrations in LNCaP by flow cytometry.Results:After DHT acts on LNCaP cells,the Ca2+ of LNCaP increased significantly(P<0.05),but the difference of the increasing degree of each index in different time points.After FLU acts on LNCaP cells,the content of Ca2+ increased significantly(P<0.05).Conclusion:The content of Ca2+ is not obvious decreased in LNCaP cell by DHT,showed the low effect on signal transduction and the apoptosis.However,the concentration of Ca2+ in LNCaP cells could be increased obviously by FLU,which leads to calcium overload and promotes cell apoptosis.

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Last Update: 2017-01-26