|Table of Contents|

The effect and mechanism of Serglycin stable interference on the sensitivity of nasopharyngeal carcinoma cells with high metastatic cells to cisplatin

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 06
Page:
856-859
Research Field:
Publishing date:

Info

Title:
The effect and mechanism of Serglycin stable interference on the sensitivity of nasopharyngeal carcinoma cells with high metastatic cells to cisplatin
Author(s):
Liang Wanning1; Wu Wenqiang2; Chen Wenhua3; Lan Zhong4
1.Pharmaceutical Department;2.Oncology Department,Zhaoqing People's Hospital,Guangdong Zhaoqing 526040,China;3.Pharmaceutical Department,Southern Medical University,Guangdong Guangzhou 510515，China;4.Clinical Pharmaceutics Department,Maoming People's Hospital,Guangdong Maoming 525000，China.
Keywords:
Serglycin; stable interference; nasopharyngeal carcinoma; cisplatin sensitivity
PACS:
R739.6
DOI:
10.3969/j.issn.1672-4992.2017.06.005
Abstract:
Objective:To observe the effect of Serglycin on the stability of high metastatic cells of nasopharyngeal carcinoma with high sensitivity to cisplatin,and to explore the mechanism of interference.Methods:Serglycin stable interference of nasopharyngeal carcinoma with high metastatic cell lines were divided into three groups:experimental group 5-8f KDA and 5-8f KDC and control group 5-8f scrambled.To detect Serglycin protein expression,to observe the parental and stable plant cell morphology.MTT assay was used to detect the absorbance,draw the curve of the proliferation under the effect of cisplatin.To detect cell viability,stem cell gene expression,and content of Vimentin and E-cadherin.Results:In the experimental group,the expression of Serglycin gene was inhibited.The Serglycin gene expression in the control group was more.The difference was significant,with statistical significance(P＜0.05).The secreted Serglycin protein in the experimental group was significantly decreased,and the expression of Serglycin in the control group was higher.The cell morphology of the stable group was round or polygon,and the control group cells were spindle shaped.MTT was found to be significantly faster in the control group than in the third day after cisplatin,and there was statistical significance(P＜0.05).Compared with the 5-8f scrambled group,the cell survival rate of the experimental group was significantly decreased,and the cell survival rate was statistically significant(P＜0.05).In stable group,the expression of gene expression was significantly decreased,and the difference was significant(P＜0.05).Serglycin can promote the expression of Vimentin protein and inhibit the expression of E-cadherin protein in tumor tissue.Conclusion:Serglycin can increase the sensitivity of nasopharyngeal carcinoma cells with high metastatic cells to cisplatin.

References:

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广东省肇庆市科技局创新计划项目(编号：2016040301-01)
Last Update: 2017-01-26