|Table of Contents|

Effect of miR-126 overexpression on the growth of human lung cancer 95D cells in vitro

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 05
Page:
701-705
Research Field:
Publishing date:

Info

Title:
Effect of miR-126 overexpression on the growth of human lung cancer 95D cells in vitro
Author(s):
Lei Liangyu12Zhao Juanjuan12Chen Chao12Xu Lin12
1.Department of Immunology and the Talent Base of Biological Treatment,Zunyi Medical College,Guizhou Zunyi 563000,China;2.The Key Laboratory of Department of Educationin,Guizhou Zunyi 563000,China.
Keywords:
miRNA-126eukaryotic expressionlung cancer95D cell
PACS:
R734.2;R730.54
DOI:
10.3969/j.issn.1672-4992.2017.05.007
Abstract:
Objective:To explore the effect of miR-126 overexpression on the growth of human lung cancer 95D cells in vitro.Methods:pcDNA3.1(-)-pri-miR-126 vector (named p-miR-126) was successfully constructed by using PCR technique.p-miR-126 was transiently transfected into 95D cells and the expression level of miR-126 was determined by TaqMan probe before and after transfection.The proliferation ability of 95D cells were detected by CCK-8 assay and the migration of 95D cells in vitro were determined by scratch assay.In addition,the level of AKT and phosphorylated AKT were analyzed by Western blot assay.Results:pcDNA3.1-miR-126 eukaryotic expression vector was successfully constructed which can upregulation of miR-126 expression (P<0.05).The proliferation and migration of 95D cells in vitro was inhibited (P<0.05).Meanwhile,the level of phosphorylation AKT was significantly decreaced.Conclusion:Overexpression of miR-126 could inhibit the growth of human lung cancer cells effectively in vitro,which may be related to the changes of AKT signaling pathway.

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Memo

Memo:
国家自然科学基金(编号:31370918);教育部“新世纪优秀人才计划”项目资助 (编号:NCET-12-0661);贵州省教育厅特色重点实验室建设项目资助(黔教合KY字[2014]212)
Last Update: 2017-01-26