|Table of Contents|

Influence from Netrin-4 to ERK/MAPK signal pathway in GBM cell lines

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 05
Page:
697-700
Research Field:
Publishing date:

Info

Title:
Influence from Netrin-4 to ERK/MAPK signal pathway in GBM cell lines
Author(s):
Li Li1; Teng Chong1; Liu Jiayin2; Lv Yanju1; Song Xiaowei1; Jin Yinghua1; Liu Ying1; Shen Weixi1; Huang Dayong1; Xin Tao1; Jiang Qiuying1
1.Department of Oncology，2nd Hospital，Harbin Medical University，Heilongjiang Harbin 150080，China；2.The Department of Radiotherapy，Cancer Hospital of Heilongjiang Province，Heilongjiang Harbin 150001，China.
Keywords:
glioblastoma; Netrin-4; signal pathway; ERK; cell senescence
PACS:
R730.264
DOI:
10.3969/j.issn.1672-4992.2017.05.006
Abstract:
Objective：To investigate the effect of different concentrations of Netrin-4 on the phosphorylation of ERK in GBM cells were detected by Western blot，and the effect of NTN4 on ERK/MAPK signaling pathway in GBM cells were discussed.Methods：U251MG cells and U87MG cells were cultured in vitro.The target protein was extracted from the cells，and the levels of phosphorylated ERK were detected by Western blot (50ng/ml-0，10min/20min，30min，1h，2h，3h；5ng/ml-0，10min/20min，30min，1h，2h，3h).Results：After the addition of U251MG，the phosphorylation of ERK in NTN4 cells and U87MG cells increased，and in U251MG cells，the level of phosphorylated at 1h was the highest after added 5ng/ml NTN4.The level of phosphorylated ERK was highest at 10min after added 50ng/ml NTN4 in U251MG cells.Meanwhile，the level of phosphorylated ERK was the highest at 10min after added either concentration in U87MG cells.Conclusion：Netrin-4 activates the ERK/MAPK signaling pathway in glioblastoma cell lines，probably which protects GBM cell senescence clarifies the mechanism.

References:

［1］Staquicini FI，Dias-Neto E，Li J，et al.Discovery of a functional protein complex of netrin-4，laminin γ1 chain，and integrinα6β1 in mouse neural stem cells［J］.Proc Natl Acad Sci USA，2009，106：2903-2908.
［2］Dakouane-Giudicelli M，Brouillet S，Traboulsi W，et al.Inhibition of human placental endothelial cell proliferation and angiogenesis by netrin-4［J］.Placenta，2015，36(11)：1260-1265.
［3］Han Y，Shao Y，Liu TT，et al.Therapeutic effects of topical netrin-4 in a corneal acute inflammatory model［J］.Int J Ophthalmol，2015，8(2)：228-233.
［4］Lejmi E，Bouras I，Camelo S，et al.Netrin-4 promotes mural cell adhesion and recruitment to endothelial cells［J］.Vasc Cell，2014，6(1)：1.
［5］Hoelzinger DB，Mariani L，Weis J，et al.Gene expression profile of glioblastoma multiforme invasive phenotype points to new therapeutic targets［J］.Neoplasia，2005，7：7-16.
［6］Yuan Y，Leszczynska M，Konstantinovsky S，et al.Netrin-4 is upregulated in breast carcinoma effusions compared to corresponding solid tumors［J］.Diagn Cytopathol，2011，38(8)：562-566.
［7］Lv B，Song C，Wu L，Zhang Q，et al.Netrin-4 as a biomarker promotes cell proliferation and invasion in gastric cancer［J］.Oncotarget，2015，6(12)：9794-9806.
［8］Zhang J，Zheng F，Yu G，et al.miR-196a targets netrin 4 and regulates cell proliferation and migration of cervical cancer cells［J］.Biochem Biophys Res Commun，2013，440(4)：582-588.
［9］Eveno C，Contreres JO，Hainaud P，et al.Netrin-4 overexpression suppresses primary and metastatic colorectal tumor progression［J］.Oncol Rep，2013，29(1)：73-78.
［10］Larrieu-Lahargue F，Welm AL，Thomas KR，et al.Netrin-4 induces lymphangiogenesis in vivo［J］.Blood，2010，115：5418-5426.
［11］Eveno C，Broqueres-You D，Feron JG，et al.Netrin-4 delays colorectal cancer carcinomatosis by inhibiting tumor angiogenesis［J］.Am J Pathol，2011，178(4)：1861-1869.
［12］Nacht M，St Martin TB，Byrne A，et al.Netrin-4 regulates angiogenic responses and tumor cell growth［J］.Exp Cell Res，2009，315：784-794.
［13］Velarde MC，Demaria M，Campisi J.Senescent cells and their secretory phenotype as targets for cancer therapy［J］.Interdisciplinary Topics in Gerontology，2013，38：17-27.
［14］Collado M，Seryano M.The power and the promise of oncogene-induced senescence markers［J］.Nature Reviews Cancer，2006，6：472-476.
［15］Li L，Hu Y，Ylivinkka I，et al.Netrin-4 protects glioblastoma cells from Temozolomide induced senescence［J］.Plos One，2013，8(11)：e80363.
［16］Hoelzinger DB，Mariani L，Weis J，et al.Gene expression profile of glioblastoma multiforme invasive phenotype points to new therapeutic targets［J］.Neoplasia，2005，7：7-16.
［17］Marie-Jose′e Boucher，Dominique Jean，Anne Ve′zina，et al.Dual role of MEK/ERK signaling in senescence and transformation of intestinal epithelial cell［J］.Am J Physiol Gastrointest Liver Physiol，2004，286：G736-G746.
［18］Cirulli V，Yebra M.Netrins：beyongd the brain［J］.Nat Rev Mol Cell Biol，2007，8：296-306.
［19］Yizhou Hu，Ylivinkka I，Chen P，et al.Netrin-4 Promotes glioblastoma cell proliferation through integrinβ4 signaling［J］.Neoplasia，2012，14(3)：219-227.
［20］Wilson BD，Ii M，Park KW，et al.Netrins promote developmental and therapeutic angiogenesis［J］.Science，2006，313：640-644.
［21］Lejmi E，Leconte L，Pedron-Mazoyer S，et al.Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B［J］.Proc Natl Acad Sci USA，2008，105：12491-12496.

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黑龙江省教育厅科学技术研究项目（编号：12541451）
Last Update: 2017-01-26