|Table of Contents|

Preliminary study on expression and biological functions of miR-148b in acute myeloid leukemia patients and cell lines

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 03
Page:
340-344
Research Field:
Publishing date:

Info

Title:
Preliminary study on expression and biological functions of miR-148b in acute myeloid leukemia patients and cell lines
Author(s):
Wang Xiaoxue; Fu Wei; Liang Ying; Li Yan
Department of Hematology，the First Hospital of China Medical University，Lianning Shenyang 110001，China.
Keywords:
miR-148b; acute myeloid leukemia; proliferation; apoptosis; cell cycles
PACS:
R733.71
DOI:
10.3969/j.issn.1672-4992.2017.03.002
Abstract:
Objective：To detect the expression level of miR-148b in acute myeloid leukemia and AML cell lines.A preliminary study on the biological function of miR-148b in AML was also conducted.Methods：56 patients with AML and 20 cases with non-malignant blood disease were collected.AML cell lines U937，THP-1 and Kasumi-1 were cultured.Expression level of miR-148b was detected by RT-PCR.miR-148b mimics/inhibitor respectively transfected U937 cell lines and Kasumi-1 cell lines.U937 cell and Kasumi-1 cell proliferation were detected by CCK-8 method.Flow cytometry was used to detect cell apoptosis and cell cycle.Results：miR-148b was significantly decreased in AML patients and AML cell lines compared with non-malignant hematologic disease control group.miR-148b mimics were transfected into U937 cell line.U937 cells were blocked in G0-G1 phase，proliferation was inhibited and apoptosis was increased after transfection.miR-148b inhibitor were transfected into Kasumi-1 cell lines，showing that cells in G0-G1 were reduced，proliferation increased and apoptosis was decreased.Conclusion：Expression of miR-148b was significantly lower in AML patients and cell lines than normal control.miR-148b plays a role of tumor suppressor genes in AML.Over expression of miR-148b can inhibit cell proliferation，promote apoptosis.

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