|Table of Contents|

Effect of miR-296 on biological behavior of pancreatic cancer cells in vitro

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 03
Page:
335-339
Research Field:
Publishing date:

Info

Title:
Effect of miR-296 on biological behavior of pancreatic cancer cells in vitro
Author(s):
Yin Jian1; 3; Zhu Guanghui2; 3; Shan Yuanzhou2; 3; Zhang Ming1; 2; 3
1.Nantong University Graduate School of Medicine，Jiangsu Nantong 226001，China；2.Department of General Surgery，Fengxian Hospital of Southern Medical University，Shanghai 201499，China；3.Department of General Surgery，Shanghai Fengxian District Central Hospital，Shanghai 201499，China.
Keywords:
miR-296-5p; pancreatic cancer; metastasis
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2017.03.001
Abstract:
Objective：To explore the effect of microRNA-296-5p （miR-296-5p） on the biological behavior of pancreatic cancer cells．Methods：The pancreatic cancer cell lines were transiently transfected with miR-296-5p Agomir and miR-296-5p Antagomir.Cell proliferation was evaluated using the Cell Counting Kit-8 (CCK-8) assay and apoptosis was assessed by flow cytometry.Adhesion was evaluated by cell adhesion experiments.Invasionand migration were measured by Transwell chamber assays.Results：The overexpression of miR-296-5p inhibited proliferation abilities as shown in the cells transfected with the miR-296-5p Agomir,in additional，the miR-296-5p accelerated apoptosis and enhanced adhension.The overexpression of miR-296-5p inhibited invasion and migration abilities，as shown in the cells transfected with the miR-296-5p Agomir.On the contrary，when transfecting miR-296-5p Antagomir，the results were completely opposite.Conclusion：The experiment confirmed miR-296-5p can influence biological behavior of pancreatic cancer cells in vitro，and inhibited metastasis.

References:

［1］Siegel RL，Miller KD，Jemal A.Cancer statistics 2016［J］.CA Cancer J Clin，2016，66(1)：7-30.
［2］Díaz-López A，Moreno-Bueno G，Cano A.Role of microRNA in epithelial to mesenchymal transition and metastasis and clinical perspectives［J］.Cancer Manag Res，2014，6：205-216.
［3］Yates LA，Norbury CJ，Gilbert RJ.The long and short of microRNA［J］.Cell，2013，153(3)：516-519.
［4］Rothschild SI.microRNA therapies in cancer［J］.Mol Cell Ther，2014，2：7.
［5］Lee KH，Lin FC，Hsu TI，et al.MicroRNA-296-5p (miR-296-5p) functions as a tumor suppressor in prostate cancer by directly targeting Pin1［J］.Biochimica et Biophysica Acta，2014，1843(9)：2055-2066.
［6］Savi F，Forno I，Faversani A，et al.miR-296/Scribble axis is deregulated in human breast cancer and miR-296 restoration reduces tumor growth in vivo［J］.Clin Sci (Lond)，2014，127(4)：233-242.
［7］Vaira V，Faversani A，Martin NM，et al.Regulation of lung cancer metastasis by Klf4-Numb-like signaling［J］.Cancer Res，2013，73(8)：2695-2705.
［8］Jiao LR，Frampton AE，Jacob J，et al.MicroRNAs targeting oncogenes are down-regulated in pancreatic malignant transformationfrom benign tumors［J］.PLoS ONE，2012，7：e32068.
［9］Yu S，Lu Z，Liu C，et al.miRNA-96 suppresses kras andfunctions as a tumor suppressor gene in pancreatic cancer［J］.Cancer Res，2010，70：6015-6025.
［10］Zhao WG，Yu SN，Lu ZH，et al.The miR-217 microRNA functions as a potentialtumor suppressor in pancreatic ductal adenocarcinoma by targeting kras［J］.Carcinogenesis，2010，31(10)：1726-1733.
［11］Dong Q，Li C，Che X，et al.MicroRNA-891b is an independent prognostic factor of pancreatic cancer by targeting Cbl-b to suppress the growth of pancreatic cancer cells［J］.Oncotarget，2016，32:11001.
［12］Ouyang H，Gore J，Deitz S，et al.microRNA-10b enhances pancreatic cancer cell invasion by suppressing TIP30 expression and promoting EGF and TGF-β actions［J］.Oncogene，2014，33(38)：4664-4674.
［13］Liu R，Zhang H，Wang X，et al.The miR-24-Bim pathway promotes tumor growth and angiogenesis in pancreatic carcinoma［J］.Oncotarget，2015，6(41)：43831-43842.
［14］Xia X，Zhang K，Cen G，et al.MicroRNA-301a-3p promotes pancreatic cancer progression via negative regulation of SMAD4［J］.Oncotarget，2015，6(25)：21046-21063.
［15］Yu J，Li A，Hong SM，et al.MicroRNA alterations of pancreatic intraepithelial neoplasias［J］.Clin Cancer Res，2012，18(4)：981-992.

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国家自然科学基金青年科学基金项目(编号：81402377)
Last Update: 2016-12-29