|Table of Contents|

Detection and clinical significance of Survivin gene in peripheral blood

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 02
Page:
283-286
Research Field:
Publishing date:

Info

Title:
Detection and clinical significance of Survivin gene in peripheral blood
Author(s):
Wang Hanmin; Zheng Yi
Department of Laboratory,Hubei Provincial Hospital of Traditional Chinese Medicine,Hubei Wuhan 430061,China.
Keywords:
Survivin gene; fluorescence quantitative PCR; cancer; peripheral blood
PACS:
R730.3
DOI:
10.3969/j.issn.1672-4992.2017.02.033
Abstract:
Objective:To establish a specific detection of retroviral Survivin mRNA expression in peripheral blood of double FQ-PCR method.Methods:To design specific primers,fluorescent probes,Survivin gene expression before and after testing in healthy people and cancer patients was tested.Results:Established the standard curve of GADPH,Survivin gene,and there was a good correlation R2 were 0.998 3 and 0.996 0.The housekeeping gene GADPH,50 healthy people and 47 patients with cancer（after treatment) in vivo expression（respectively:6.45×105,6.57 × 105,6.74×105 copies/ml) was not statistically different.Survivin gene,was not expressed in healthy people（Ct values≥28) in cancer patients（before treatment) detected in 91.5%（43/47) expression,the expression level of 5.35×105copies/ml，cancer patients（after treatment) detected 46.8%（22/47),the expression level of 3.18×105copies/ml.Conclusion:This study was established to detect survivin dual fluorescence quantitative polymerase chain reaction（PCR) in peripheral blood,this method in healthy people and patients with cancer and evaluation of different methods for treating cancer,on the effectiveness of cancer treatment evaluation has a good application.

References:

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Last Update: 2016-12-01