|Table of Contents|

Inhibitory effect of HOXA9 gene on migration and invasion ability in hepatocellular carcinoma cell HepG2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 01
Page:
29-33
Research Field:
Publishing date:

Info

Title:
Inhibitory effect of HOXA9 gene on migration and invasion ability in hepatocellular carcinoma cell HepG2
Author(s):
Ge Yuanxun1; Gong Li1; Zhu Shaojun1; Han Xiujuan1; Yao Li1; Zhang Jiarui1; Lan Miao1; Peng Shiyuan2; Li Yanhong2; Zhang Wei1
1.Department of Pathology；2.Department of Gynecology and Obstetrics，Tangdu Hospital，The Fourth Military Medical University，Shaanxi Xi'an 710038，China.
Keywords:
hepatocellular carcinoma; HOXA9; migration; invasion
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2017.01.008
Abstract:
Objective：To investigate the inhibitory effects of HOXA9 gene on migration and invasion of hepatocellular carcinoma cell line HepG2.Methods：Eukaryotic vector expressing HOXA9 gene and empty vector were transfected into hepatocellular carcinoma cell line HepG2 by EndoFectinTM-Max transfection reagents.Liposome mediated method was used to transfect siRNA sequence targeting HOXA9 and control sequence into HepG2 cells.HOXA9 expression levels after transfection were detected by using Real-time PCR and Western blot methods.The tumor cell abilities of migration and invasion after transfection were measured by wound scratch and transwell assays.Results：The mRNA and protein levels of HOXA9 were significantly increased after transfection of Eukaryotic vector expressing HOXA9 gene.Wound scratch assay revealed the ability of cell mobility was significantly reduced after transfecting of HOXA9 gene，and Transwell assay showed the number of migratory cells in HOXA9 over-expressing group was significantly reduced.While after transfecting siRNA sequence targeting HOXA9，the mRNA and protein levels of HOXA9 were significantly reduced.Wound scratch assay revealed the ability of cell mobility in siRNA group was significantly increased，and Transwell assay showed the number of migratory cells in siRNA group was significantly increased.Conclusion：HOXA9 gene inhibit migration and invasion abilities of HepG2 cell，which can provide an experiment basis for investigating effects of HOXA9 in the occurrence and development of hepatocellular carcinoma.

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Memo

Memo:
国家自然科学基金面上项目（编号：81372226）；国家重大基础研究计划（973计划）（编号：2015CB553703）；陕西省创新工程（编号：2011KTCL03-11）
Last Update: 2016-12-01