|Table of Contents|

The effects of microRNA-9 on proliferation and apoptosis of osteosarcoma MG-63 cells and correlated mechanisms

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 01
Page:
25-29
Research Field:
Publishing date:

Info

Title:
The effects of microRNA-9 on proliferation and apoptosis of osteosarcoma MG-63 cells and correlated mechanisms
Author(s):
Wei Qiuye1; Wang Yingbin1; Nong Feng1; Zhu Jie1; Tan Haiyan2; Zou Chongqi3; Lai Changsheng3
1.Graduate College of Youjiang Medical University for Nationalities，Guangxi Baise 533000，China；2.Luoding Red Cross Hospital，Guangdong Luoding 527200，China；3.Yulin Red Cross Hospital，Guangxi Yulin 537000，China.
Keywords:
osteosarcoma; miRNA-9; transfection; proliferation; apoptosis; CXCR4
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2017.01.007
Abstract:
Objective：To study the function of miRNA-9 on osteosarcoma MG-63 cells proliferation and apoptosis and correlated mechanisms by improving the expression of miRNA-9.Methods：Osteosarcoma MG-63 cells were randomly divided into three groups：Experimental group(transfected with miRNA-9 Oligo)，control group(transfected with miRNA negative contrat）and blank group(transfected nothing).Detected the expression of miRNA-9，CXCR4 by real time PCR.Detected the CCK value(OD）at 24h，48h，72h and 96h，then observed the cell growth curve.The apoptosis was measured by flow cytometry.Results：Compared with negative control group and blank group，the miRNA transfection group was higher expressed on miRNA-9，osteosarcoma cells showed lower cell proliferation efficiency and higher cell apoptosis rotio on miRNA-9 higher expression groups.Conclusion：CXCR4 gene involved in the miRNA-9 inhition of osteosarcoma MG-63 cell proliferation，and promote cell apoptosis.

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Last Update: 2016-12-01