|Table of Contents|

The study on drug resistance of side population cells of cervical carcinoma in vitro

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 01
Page:
21-25
Research Field:
Publishing date:

Info

Title:
The study on drug resistance of side population cells of cervical carcinoma in vitro
Author(s):
Ji Rui1; Lu Hong1; Jin Hua2; He Aiqin1
1.Department of Gynecologic Oncology；2.Department of Laboratory，Nantong Tumor Hospital，Jiangsu Nantong 226361，China.
Keywords:
cervical carcinoma; side population; ABCG2; ABCC2; drug resistance
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2017.01.006
Abstract:
Objective：To observe the expression of ABCG2 and ABCC2 in side population cells(SPCs） and non-side population cells(NSPCs） originated from Hela clone of cervical carcinoma which were treated by cisplatin(DDP）and to explore the difference of drug resistance between the two cell groups.Methods：SPCs and NSPCs were screened from Hela clone of human cervical carcinoma by using Hoechst 33342-stain method and flow cytometry.The expression of ABCG2 and ABCC2 in SPCs and NSPCs were detected by Real-Time PCR technology，and the ratio of apoptosis in the two group cells were calculated.Results：Expression of ABCG2 and ABCC2 in SPCs was higher than NSPCs，but there was no significant difference between the two groups(P>0.05).Expression of ABCG2 and ABCC2 rise in each group when the cells was treated with DDP，and there had significant difference between the two groups(P<0.05).The apoptosis ratio of SPCs basesd on IC50 values had almost not changed when the cells were treated with DDP，especially on the concentration of 0.95μg/ml and 1.9μg/ml，while there had lots of apoptosis in NSPCs，in particular on the concentration of 0.95μg/ml and 1.9μg/ml of DDP.Conclusion：SPCs which were separated from Hela cell line of human cervical carcinoma has stronger drug resistance ability than NSPCs deriving from the same cell clone.The rise of ABCG2 and ABCC2 expression may responsible for the drug resistance in SPCs，basing on the cell apoptosis number decreased.

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Last Update: 2016-12-01