|Table of Contents|

The effect of DUSP10 on migration and invasion of ovarian cancer A2780 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 01
Page:
17-20
Research Field:
Publishing date:

Info

Title:
The effect of DUSP10 on migration and invasion of ovarian cancer A2780 cells
Author(s):
Peng Shiyuan1Ge Yuanxun2Yao Li2Zhang Jiarui2Wang Shumei2Lan Miao2Zhang Wei2Li Yanhong1
1.Department of Obstetrics and Gynecology;2.Department of Pathology,Tangdu Hospital,The Fourth Military Medical University,Shaanxi Xi'an 710038,China.
Keywords:
serous ovarian cancerDUSP10migrationinvasion
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2017.01.005
Abstract:
Objective:To investigate the expression of DUSP10 in serous ovarian cancer tissues and its relationship with ovarian cancer cell migration and invasion ability.Methods:Real-time quantitative PCR was performed to detect the expression of DUSP10 in serous ovarian cancer and normal fimbria of fallopian tubes.Wound healing assay and Transwell assay were used to observe the migration and invasion ability of A2780 cells by transfected DUSP10 overexpression plasmid and interference plasmid.Results:The mRNA expression of DUSP10 was significantly down-regulated in serous ovarian cancers than normal fimbria of fallopian tubes (P<0.05).The migration and invasion ability of A2780 cells were significantly inhibited after overexpression of DUSP10,however,silencing DUSP10 can promote cell migration and invasion.Conclusion:The expression of DUSP10 was significantly down-regulated in serous ovarian cancer,DUSP10 can inhibit the migration and invasion ability of ovarian cancer A2780 cells.

References:

[1]Nam EJ,Yoon H,Kim SW,et al.MicroRNA expression profiles in serous ovarian carcinoma[J].Clin Cancer Res,2008,14(9):2690-2695.
[2]Li Y,Yao L,Liu F,et al.Characterization of microRNA expression in serous ovarian carcinoma[J].Int J Mol Med,2014,34(2):491-498.
[3]Kim J,Coffey DM,Creighton CJ,et al.High-grade serous ovarian cancer arises from fallopian tube in a mouse model[J].Proc Natl Acad Sci,2012,109(10):3921-3926.
[4]Di Leva G,Croce CM.The Role of microRNAs in the tumorigenesis of ovarian cancer[J].Frontiers in Oncol,2013,2013(3):153-162.
[5]Kinose Y,Sawada K,Nakamura K,et al.The role of MicroRNAs in ovarian cancer[J].Bio Med Res Int,2014,2014(10):1-11.
[6]Theodosiou A,Smith A,Gillieron C,et al.MKP5,a new member of the MAP kinase phosphatase family,which selectively dephosphorylates stress-activated kinases[J].Oncogene,1999,18(50):6981-6988.
[7]Qian F,Deng J,Gantner B,et al.Map kinase phosphatase 5 protects against sepsis-induced acute lung injury[J].Am J Physiol-Heart,2012,302(9):866-874.
[8]Png CW,Weerasooriya M,Guo J,et al.DUSP10 regulates intestinal epithelial cell growth and colorectal tumorigenesis[J].Oncogene,2016,35(2):206-217.
[9]He G,Zhang L,Li Q,et al.miR-92a/DUSP10/JNK signalling axis promotes human pancreatic cancer cells proliferation[J].Biomedicine & Pharmacotherapy,2014,68(1):25-30.
[10]He HY,Fang WG,Zheng J,et al.Mechanism of the mitogen-activated protein kinase phosphatase-5 regulating the growth and invasion of a human prostate cancer cell line[J].National Med J China,2003,83(20):64-69.[贺慧颖,方伟岗,郑杰,等.丝裂原活化蛋白激酶磷酸酶5调节人前列腺癌细胞生长及侵袭的信号转导机制研究[J].中华医学杂志,2003,83(20):64-69.]
[11]Song M,Park Y,Ryu J,et al.Polycyclic aromatic hydrocarbon(PAH)-mediated upregulation of hepatic microRNA-181 family promotes cancer cell migration by targeting MAPK phosphatase-5,regulating the activation of p38MAPK[J].Toxicol Applied Pharmacol,2013,273(1):130-139.
[12]Erickson BK,Conner MG,Landen CN,et al.The role of the fallopian tube in the origin of ovarian cancer[J].Am J Obst Gynecol,2013,209(5):409-414.
[13]Yue J,Liu F,Li YH,et al.Expression and clinical significance of DUSP10 and BZW1 in serous ovarian tumors[J].Modern Oncology,2016,24(10):1626-1630.[岳娟,刘菲,李艳红,等.DUSP10 和 BZW1 在卵巢浆液性肿瘤中的表达及临床意义[J].现代肿瘤医学,2016,24(10):1626-1630.]

Memo

Memo:
国家重大基础研究计划(973计划)(编号:2015CB553703);卫生部医药卫生科技发展研究中心课题(编号:W2013FZ13);陕西省社会发展攻关计划项目(编号:2014K11-01-01-25)
Last Update: 2016-12-01