|Table of Contents|

The study of apoptosis and mechanism induced by Zanthoxylum bungeanum Maxim extracts in hepatocellular carcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 01
Page:
14-17
Research Field:
Publishing date:

Info

Title:
The study of apoptosis and mechanism induced by Zanthoxylum bungeanum Maxim extracts in hepatocellular carcinoma cells
Author(s):
Zhao Xiangxuan; Zhang Chaoya; Wen Feng; Lu Zaiming
Department of Radiology，Shengjing Hospital of China Medical University，Liaoning Shenyang 110004，China.
Keywords:
Zanthoxylum bungeanum Maxim extract; HepG2; liver cancer; apoptosis; signal transduction
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2017.01.004
Abstract:
Objective：To investigate the effects of Zanthoxylum bungeanum Maxim extract（ZBME）-induced apoptosis in hepatocellular carcinoma cells.Methods：HepG2 cells were cultured in vitro.We set up control group and ZBME-treated group.Cells untreated or treated with various conditions were observed under contrast microscope to show apoptosis and cell growth inhibition.MTT assay was performed to measure cell viability.Western blotting was performed to detect apoptosis-related molecules such as Mcl-1，Survivin，Bcl-xL，Bax.FACs based on Annexin V-PI double staining was used to assess apoptosis ratio.Results：ZBME induced apparent apoptosis and cell growth inhibition in HepG2 cells.Apoptosis and cell growth inhibition induced by ZBME may be mediated by down-regulation of anti-apoptotic proteins Mcl-1，Survivin and Bcl-xL，and up-regulation of pro-apoptotic protein Bax.Conclusion：ZBME effectively exerted apoptotic cytotoxicity in liver cancer cells by regulating apoptotic protein expression.

References:

［1］Peck-Radosavljevic M.Drug therapy for advanced-stage liver cancer［J］.Liver Cancer，2014，3：125-131.
［2］Woo HY，Heo J.Sorafenib in liver cancer［J］.Expert Opin Pharmacother，2012，13：1059-1067.
［3］Hassan M，Watari H，AbuAlmaaty A，et al.Apoptosis and molecular targeting therapy in cancer［J］.Biomed Res Int，2014，2014：150845.
［4］Zang LQ，Hu F，Wei M，et al.The anti-tumor effect of Zanthoxylunm Maxim and its mechanism of essential oil of Zanthoxylunm Maxim［J］.J Snake，2006，18(3)：183-186.
［5］Li PA，Li XL，Zhang L.Study on the effect of Pericarpium Zanthoxyli extracts on proliferation and apoptosis of SGC-790l cells［J］.J Anhui Agricultural Sci，2011，39(20)：12091-12092.［李品艾，李晓莉，张玲.花椒提取物对人胃癌细胞增殖及凋亡作用的研究［J］.安徽农业科学，2011，39(20)：12091-12092.］
［6］Huang HC，Wang RY，Zhou WM.Lethal effect of Zanthoxylunm Maxim Volatile oil on pheochromocytoma cells［J］.Heilongjiang Med J，2010，23(4)：514-515.［黄海潮，王如意，周伟民.花椒挥发油对嗜铬细胞瘤细胞的杀伤作用［J］.黑龙江医药，2010，23(4)：514-515.］
［7］Zhang DS，Zhong QX，Song XM，et al.Study on the chemical components antimicrobial and antitumor activities of the essential oil from the leaves of Zanthoxylum avicennae［J］.J Chin Med Materials，2012，35(8)：1263-1267.［张大帅，钟琼芯，宋鑫明，等.簕欓花椒叶挥发油的GC-MS分析及抗菌抗肿瘤活性研究［J］.中药材，2012，35(8)：1263-1267.］
［8］Yuan TN，Wang YL，Wang JZ.Primary study of the anti-tumor effect and its mechanism of Zanthoxylum in vivo and in vitro［J］.Li Shizhen Medicine Materia Medica Res，2008，19(12)：2915-2916.
［9］Yang L，Shao WB，Yu AH，et al.Anti-tumor activities of total polysaccharides from Zanthoxylum Dissitum Pericarp［J］.Acta Chin Med Pharmacol，2012，40(5)：34-37.［杨林，邵文斌，于爱红，等.蚬壳花椒果皮多糖抗肿瘤活性研究［J］.中医药学报，2012，40(5)：34-37.］
［10］Xue T，Huang JR，Li HL.Research progress on domestic and foreign pepper by-prod|ucts［J］.China Condiment，2013，38(12)：106-110.
［11］Wang HW.Precious plant Resources-Zanthoxylum［J］.Tianjin Agricultural Sci，2011，17(2)：126-128.［王宏伟.珍贵植物资源-花椒［J］.天津农业科学，2011，17(2)：126-128.］
［12］Yuan TN，Xiao CY，Wang JZ,et al.Primary study of the anti-cervical cancer effect on Caski cells and its mechanism of Zanthoxylum［J］.Li Shizhen Medicine Materia Medica Res，2009，20(5)：1119-1120.［袁太宁，肖长义，汪鋆植，等.花椒抗宫颈癌Caski细胞作用及其机制的初步研究［J］.时珍国医国药，2009，20(5)：1119-1120.］

Memo

Memo:
国家自然科学基金(编号：31371425)；国家自然科学基金(编号：31240025);辽宁省自然科学基金(编号：2013023056)
Last Update: 2016-12-01