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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2017 01

Page:

9-13

Research Field:

Publishing date:

Info

Title:

The matrine reverse multidrug resistance on breast cancer MCF-7/ADR cell line and have an influence on downstream factors of PI3K/AKT signal pathway

Author(s):

Zhou Binggang¹; Wei Changsheng¹; Zhang Song³; Zhang Zhi³; Wang Jian⁴

1.Department of Tumor Surgery，The First People's Hospital of Yinchuan，Ningxia Yinchuan 750002，China；2.Department of Breast Cancer Surgery，Gansu Provincal Cancer Hospital，Gansu Lanzhou 730050，China；3.Graduate of School,Ningxia Medical University，Ningxia Yinchuan 750004,China;4.Pharmacy Center，Ningxia Autonomous Region People's Hospital，Ningxia Yinchuan 750005，China.

Keywords:

matrine; MCF-7/ADR; multidrug resistance; PI3K/AKT signal pathway

PACS:

R737.9

DOI:

10.3969/j.issn.1672-4992.2017.01.003

Abstract:

Objective：To explore the mechanism of matrine in reversaling multidrug resistance on breast cancer cells and have an influence on regulating downstream factors of PI3K/AKT signal pathway.Methods：MTT assay was used to measure the inhibition rate of Matrine on MCF-7/ADR cells after 24 hours，Real-time RT-PCR and Western blotting were used to evaluate the mRNA expression and the protein level of Bcl-2，Bax，Caspase-3，PARP，GSK-3β and p65 in MCF-7/ADR cells.Results：Real-time RT-PCR showed that 0.3，0.6 and 1.2g/L groups of matrine were compared with control group，the multidrug resistance genes expression amount of Bcl-2，p65 reduced gradually，but the gene expression differences of Bax,GSK-3β and PARP increased gradually and gene expression differences of Caspase-3 was not obvious.The gene of Bcl-2，p65 which were intervened by 0.6g/L matrine group and the difference of 0.05μmol/L MK2206 group had no significant difference at the same inhibition radio.Western blotting showed when 0.3，0.6 and 1.2g/L groups of matrine were compared with control group，the protein expression of Bcl-2 and p65 reduced gradually.But that of Bax,GSK-3β and PARP increased gradually and that of Caspase-3 didn't changed obviously.Matrine group was more likely to reduce the expression of Bcl-2 and p65 and increase the expression of Bax,GSK-3β and PARP at the same inhibition radio of matrine(0.6g/L）and MK2206(0.05μmol/L).Conclusion：The effects of matrine reversaling multidrug resistance is probably related to regulating the downstream apoptosis factors of PI3K/AKT signal pathway through AKT target gene.

References:

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国家自然科学基金（编号：81260388）

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Last Update: 2016-12-01