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The role of silybin in inhibiting proliferation and promoting apoptosis of breast cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

2016 06
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The role of silybin in inhibiting proliferation and promoting apoptosis of breast cancer cells
Zhou DayongHai Yongwei
Department of General Surgery,the 82nd Hospital of PLA,Jiangsu Huai'an 223001,China.
silybinPUMAbreast cancerMCF7 cells
Objective:To investigate the role of silybin in inhibiting proliferation and promoting apoptosis of breast cancer MCF7 cells.Methods:The cells were divided into control group and SIL groups (10,20,40mg/L),each group was treated for 24,48 and 72h.MTT method was used to detect the survival rate of cells.TUNEL was used to detect the apoptotic rate of cells.Special kits were used to detect the activity of caspase3/7.Western bolt method was used to detect the expression of PUMA.Results:The MTT results show that SIL could inhibit the proliferation of MCF7 cells on dose and time-depend manner,the effect of 40mg/L SIL (72h) was the most obvious.After being treated with SIL (48h),the apoptotic rate of cells and the activity of caspase3/7 increased significantly.In addition,PUMA protein increased significantly in SIL treated group.Conclusion:Silybin can inhibit proliferation and promote apoptosis of breast cancer MCF7 cells.PUMA may play a role in the apoptosis of breast cancer MCF7 cells induced by silybin.


[1]Wei Q,He W,Lu Y,et al.Effect of the tumor suppressor gene ING4 on the proliferation of MCF-7 human breast cancer cells[J].Oncol Lett,2012,4(3):438-442.
[2]Barbero-Becerra VJ,Méndez-Sánchez N.Silybin/Silymarin treatment in chronic hepatitis C[J].Ann Hepatol,2012,11(5):731-733.
[3]Di Cesare Mannelli L,Zanardelli M,Failli P,et al.Oxaliplatin-induced neuropathy:oxidative stress as pathological mechanism.Protective effect of silibinin[J].J Pain,2012,13(3):276-284.
[4]S Fan,M Qi,Y Yu,et al.p53 activation plays a crucial role in silibinin induced ROS generation via PUMA and JNK[J].Free Radical Res,2012,46(3):310-319.
[5]Vargas-Mendoza N,Madrigal-Santillán E,Morales-González A,et al.Hepatoprotective effect of silymarin[J].World J Hepatol,2014,6(3):144-149.
[6]Kauntz H,Bousserouel S,Gosse F,et al.Silibinin,a natural flavonoid,modulates the early expression of chemoprevention biomarkers in a preclinical model of colon carcinogenesis[J].Int J Oncol,2012,41(3):849-854.
[7]Kauntz H,Bousserouel S,Gossé F,et al.The flavonolignan silibinin potentiates TRAIL-induced apoptosis in human colon adenocarcinoma and in derived TRAIL-resistant metastatic cells[J].Apoptosis,2012,17(8):797-809.
[8]Zhang P,Liu SS,Ngan HY.TAp73-mediated the activation of C-Jun N-terminal kinase enhances cellular chemosensitivity to cisplatin in ovarian cancer cells[J].PLoS One,2012,7(8):e42985.
[9]Yoon CH,Rho SB,Kim ST,et al.Crucial role of TSC-22 in preventing the proteasomal degradation of p53 in cervical cancer[J].PLoS One,2012,7(8):e42006.
[10]Yousefi M,Ghaffari SH,Soltani BM,et al.Therapeutic efficacy of silibinin on human neuroblastoma cells:Akt and NF-κB expressions may play an important role in silibinin-induced response[J].Neurochem Res,2012,37(9):2053-2063.
[11]Wang YX,Cai H,Jiang G,et al.Silibinin inhibits proliferation,induces apoptosis and causes cell cycle arrest in human gastric cancer MGC803 cells via STAT3 pathway inhibition[J].Asian Pac J Cancer Prev,2014,15(16):6791-6798.
[12]Akhtar R,Ali M,Mahmood S,et al.Anti-proliferative action of silibinin on human colon adenomatous cancer HT-29 cells[J].Nutr Hosp,2014,29(2):388-392.
[13]Duan WJ,Li QS,Xia MY,et al.Silibinin activated p53 and induced autophagic death in human fibrosarcoma HT1080 cells via reactive oxygen species-p38 and c-Jun N-terminal kinase pathways[J].Biol Pharm Bull,2011,34(1):47-53.
[14]Pardee TS.Overexpression of MN1 confers resistance to chemotherapy,accelerates leukemia onset,and suppresses p53 and bim induction[J].PLoS One,2012,7(8):e43185.
[15]Neboori HJ,Haffty BG,Wu H,et al.Low p53 binding protein 1 (53BP1) expression is associated with increased local recurrence in breast cancer patients treated with breast-conserving surgery and radiotherapy[J].Int J Radiat Oncol Biol Phys,2012,83(5):e677-683.


Last Update: 2016-01-29