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Experimental research on the effects of lycopene on the invasion and migratory ability of human non-small cell lung cancer H520 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

2016 06
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Experimental research on the effects of lycopene on the invasion and migratory ability of human non-small cell lung cancer H520 cells
Zhang Yujiang1Ma Lijie2Yang Shuiqing1Feng Jinghua1
1.The 169 Hospital,Hunan Hengyang 421000,China;2.Tangdu Hospital,Fourth Military Medical University,Shaanxi Xi'an 710032,China.
lycopenenon-small cell lung cancerinvasionmigration
Objective:To investigate the effects of lycopene on the proliferation,invasion and migratory ability of human non-small cell lung cancer H520 cells.Methods:The survival rate of H520 cells treated with varied dose of lycopene for 24,48 and 72h was detected by MTT assay.Transwell and wound healing analysis were adopted to evaluate invasion and migratory ability of H520 cells stimulated by 7μmol/L of lycopene,respectively.mRNA expression of matrix metalloproteinase (MMP)-2 and -9 was examined by real-time PCR.Results:Lycopene inhibited the proliferation of H520 cells in a time- and dose-dependent manner (P<0.05).7μmol/L of lycopene reduced the number of H520 cells invaded into the lower chamber of Transwell system (P<0.05) as well as the distance that cell migrated to heal the wound.Lycopene inhibited the mRNA expression of MMP-2 and -9 in a dose dependent manner in H520 cells.Conclusion:Lycopene inhibited the proliferation,invasion and migratory abilities of H520 cells probably through interfering the expression of MMP-2 and MMP-9.


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Last Update: 2016-01-29