|Table of Contents|

Effects and preliminary mechanism of miR-26a on cell invasion and migration in the hepatocellular carcinoma cell line HepG2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2016 06
Page:
878-881
Research Field:
Publishing date:

Info

Title:
Effects and preliminary mechanism of miR-26a on cell invasion and migration in the hepatocellular carcinoma cell line HepG2
Author(s):
Qiu Feng; Fei Jianping; Lu Yi; Jiang Baocheng
Department of General Surgery,Suzhou Kowloon Hospital,Shanghai Jiaotong University School of Medicine,Jiangsu Suzhou 215000,China.
Keywords:
miR-26a; hepatocellular carcinoma cells line HepG2; AEG-1; invasion; migration
PACS:
R73-3;R735.7
DOI:
10.3969/j.issn.1672-4992.2016.06.008
Abstract:
Objective:To explore the effect of miR-26a on the invasion and migration of hepatocellular carcinoma cell line HepG2 and its mechanism.Methods:HepG2 cells were transfected with miR-26a mimics or negative control,then transwell assay,wound healing assay were used for investigating cell invasion and migration,qRT-PCR and Western blot were performed to measure the expression of AEG-1 mRNA and protein.Results:miR-26a was upregulated after transfected with miR-26a mimics in HepG2 cells compared with NC,the ability of invasion and migration was significantly decreased (P＜0.05).Expression of AEG-1 mRNA and protein was also downregulated observably (P＜0.05).Conclusion:Overexpression of miR-26a in HepG2 cells may suppress cells invasion and migration，probably via downregulating its target gene AEG-1 expression．

References:

［1］Li SH,Guo ZX,Xiao CZ,et al.Risk factors for early and late intrahepatic recurrence in patients with single hepatocellular carcinoma without macrovascular invasion after curative resection［J］.APJCP,2013,14(8):4759-4763.
［2］Ambros V.The functions of animal microRNAs［J］.Nature,2004,431(7006):350-355.
［3］Bartel DP.MicroRNAs:genomics,biogenesis,mechanism,and function［J］.Cell,2004,116(2):281-297.
［4］Zhang J,Han C,Wu T.MicroRNA-26a promotes cholangiocarcinoma growth by activating β-catenin［J］.Gastroenterology,2012,143(1):246-256.
［5］Kato M,Goto Y,Matsushita R,et al.MicroRNA-26a/b directly regulate La-related protein 1 and inhibit cancer cell invasion in prostate cancer［J］.Int J Oncol,2015,47(2):710-718.
［6］Wang G,Sun Y,He Y,et al.miR-26a promoted by interferon-alpha inhibits hepatocellular carcinoma proliferation and migration by blocking EZH2［J］.Genetic Testing Molecular Biomarkers,2015,19(1):30-36.
［7］Farh KK,Grimson A,Jan C,et al.The widespread impact of mammalian microRNAs on mRNA repression and evolution［J］.Science,2005,310(5755):1817-1821.
［8］Xiong J.Emerging roles of microRNA-22 in human disease and normal physiology［J］.Current Molecular Med,2012,12(3):247-258.
［9］Zhu Z,Zhang X,Wang G,et al.Role of microRNAs in hepatocellular carcinoma［J］.Hepatitis Monthly,2014,14(8):e18672.
［10］Xie KL,Zhang YG,Liu J,et al.MicroRNAs associated with HBV infection and HBV-related HCC［J］.Theranostics,2014,4(12):1176-1192.
［11］Chen Y,Chen J,Liu S,et al.Plasma miR-15b-5p,miR-338-5p,and miR-764 as biomarkers for hepatocellular carcinoma［J］.Med Sci Monit,2015,21:1864-1871.
［12］Zhao N,Wang R,Zhou L,et al.MicroRNA-26b suppresses the NF-kappaB signaling and enhances the chemosensitivity of hepatocellular carcinoma cells by targeting TAK1 and TAB3［J］.Molecular Cancer,2014,13:35.
［13］Ji J,Shi J,Budhu A,et al.MicroRNA expression,survival,and response to interferon in liver cancer［J］.New Eng J Med,2009,361(15):1437-1447.
［14］Motalleb G,Gholipour N,Samaei NM.Association of the human astrocyte elevated gene-1 promoter variants with susceptibility to hepatocellular carcinoma［J］.Med Oncol,2014,31(4):916.
［15］Li S,Guo X,Ma X,et al.Expression of astrocyte elevated gene-1 closely correlates with the angiogenesis of gastric cancer［J］.Oncol Let,2014,7(5):1447-1454.
［16］Gnosa S,Shen YM,Wang CJ,et al.Expression of AEG-1 mRNA and protein in colorectal cancer patients and colon cancer cell lines［J］.J Tran Med,2012,10:109.
［17］Zhang X,Zhang N,Zhang MX.Astrocyte elevated gene-1 induces breast cancer proliferation and invasion through upregulating HER2/neu expression［J］.Chin Med J,2011,124(21):3546-3550.
［18］Shi X,Wang X.The role of MTDH/AEG-1 in the progression of cancer［J］.Int J Clin Exp Med,2015,8(4):4795-4807.
［19］Jung HI,Ahn T,Bae SH,et al.Astrocyte elevated gene-1 overexpression in hepatocellular carcinoma:an independent prognostic factor［J］.Ann Surg Treat Res,2015,88(2):77-85.

Memo

Memo:
-
Last Update: 2016-01-29