|Table of Contents|

MRP1-mediated tumor multidrug resistance

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

2016 06
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MRP1-mediated tumor multidrug resistance
Wang Xinping1Yan Lvnan2
1.Department of General Surgery,Xi'an No.4 Hospital,Shaanxi Xi'an 710004,China;2.Department of General Surgery,West China Hospital,Sichuan University,Sichuan Chengdu 610041,China.
multidrug resistancemultidrug resistance-associated proteinRNA interference
Objective:To investigate the suppression of MRP1 and MRP1 induced by small interfering RNA and the restoration of sensitivity to chemotherapeutic drugs in the multidrug-resistant hepatocellular carcinoma cell lines HepG2/MRP1.Methods:MRP1-targeted small interfering RNA duplexes were designed,composed and introduced into multidrug-resistant hepatocellular carcinoma cell lines HepG2/MRP1.The suppression of MRP1 mRNA and its gene product MRP1 was examined by RT-PCR and flow cytometry (FCM) respectively. MTT assay was performed to measure the reverse effect of small interfering RNA based on the results of IC50.Results:The overexpression of MRP1 mRNA and MRP1 was suppressed,by the introduction of small interfering RNAs,which caused sequence-specific gene silence.The level of MRP1 mRNA in the transfected HepG2/MRP1 cells reduced to (86.36±2.76) % and MRP1 to (89.38±3.76)% compared with those of the controls. The resistance to ADR was reversed by five folds,which indicated the restoration of sensitivity to drugs.Conclusion:The multidrug resistance of tumors were closely related to the high expression of MRP1. Small interfering RNA can inhibit MRP1 expression and reverse the multidrug resistance mediated by MRP1.


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Last Update: 2016-01-29