|Table of Contents|

Experimental study on inhibition of EMT and cell cycle ofgastric cancer cells by overexpression of KLF4 through Wnt/β-catenin signal pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 06
Page:
991-996
Research Field:
Publishing date:

Info

Title:
Experimental study on inhibition of EMT and cell cycle ofgastric cancer cells by overexpression of KLF4 through Wnt/β-catenin signal pathway
Author(s):
FU Yue1; LI Chunming1; LIANG Na2; LUO Qingqing3; WU Si1; ZHOU Hanghao3
1.Zunyi Medical University,Guizhou Zunyi 563000,China;2.Department of Tissue and Embryology,Zunyi Medical University,Guizhou Zunyi 563000,China;3.Department of Pathology,Affiliated Hospital of Zunyi Medical University,Guizhou Zunyi 563000,China.
Keywords:
gastric cancer; KLF4; EMT; Wnt/β-catenin signal pathway
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2023.06.002
Abstract:
Objective：To investigate the mechanism by which overexpression of KLF4 inhibits epithelial-mesenchymal transition (EMT) and cell cycle in gastric cancer cells through the Wnt/β-catenin signaling pathway.Methods:BGC-823 cells that had been successfully transfected with KLF4 expression in the previous phase of the group were divided into a KLF4 overexpression vector group (BGC823-OE group),an empty virus vector group (BGC823-NC group) and an untreated BGC-823 cell group (BGC823-Ctrl group).Western blot and qRT-PCR were performed to detect the expression levels of KLF4,EMT and Wnt signalling pathway related proteins and mRNA in the three groups of cells.CCK-8 assay,scratch assay,Transwell migration assay,plate cloning assay and flow cytometry were used to observe the proliferation ability,migration ability,clone formation ability and cell cycle changes of each group of cells.Results:The results of Western blot and qRT-PCR experiments showed that the expression levels of KLF4 protein and mRNA were significantly increased in the BGC823-OE group.The epithelial marker E-cadherin protein and mRNA expression levels were increased and the mesenchymal marker,N-cadherin protein and mRNA expression levels were decreased for epithelial-mesenchymal transition.The protein and mRNA expression levels of β-catenin and Cyclin D1,the downstream targets of the Wnt signaling pathway were reduced.The results of the CCK-8 assay showed a reduced proliferative capacity in the BGC823-OE group.The results of the scratch assay and Transwell migration assay showed a decrease in the migration ability of the BGC823-OE group.The results of the plate cloning showed reduced cloning ability in the BGC823-OE group.Flow cytometry results showed an increased proportion of G0/G1 phase and a decreased proportion of S phase in the BGC823-OE group.Conclusion:Overexpression of KLF4 may inhibit EMT and block the cell cycle of gastric cancer cells through the Wnt/β-catenin signaling pathway.

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Memo

Memo:
贵州省研究生科研基金（编号：黔教合YJSKYJJ［2021］181）;贵州省遵义市科技局联合基金资助项目［编号:遵市科合HZ字(2020)52号］
Last Update: 1900-01-01