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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 06

Page:

985-991

Research Field:

Publishing date:

Info

Title:

The study of the mechanism of BMI-1 promoting the lymphoma growth in nude mice via activating PI3K/Akt signaling pathway

Author(s):

SONG Yuanhua¹; YANG Zhen¹; LI Wenling¹; SHEN Ru¹; GAO Yan¹; FENG Zhiping²

1.Kunming Children's Hospital,the Affiliated Children's Hospital of Kunming Medical University,Yunnan Kunming 650000,China;2.Department of Nuclear Medicine,the Third Affiliated Hospital of Kunming Medical University,Yunnan Cancer Hospital,Yunnan Kunming 650000,China.

Keywords:

BMI-1; PI3K/Akt; lymphoma; tumor growth

PACS:

R733.4

DOI:

10.3969/j.issn.1672-4992.2023.06.001

Abstract:

Objective：To explore the effect of proto-oncogene BMI-1 on lymphoma growth in nude mice and the potential molecular mechanism.Methods:The murine lymphoma cells FOX-NY were cultured and treated with BMI-1 silencing and overexpression,and combined with PI3K/Akt inhibitor.Cell proliferation was detected by CCK-8 method.Animal models of lymphoma in nude mice were established by axillary inoculation of murine lymphoma FOX-NY,silent BMI-1 FOX-NY (BMI-1--FOX-NY) and overexpressed BMI-1 FOX-NY(BMI-1+-FOX-NY) cells.The nude mice were divided into control group,plasmid no-load group,BMI-1--FOX-NY group and BMI-1+-FOX-NY group.The body weight,tumor volume and tumor mass of nude mice were detected.The expression of BMI-1 mRNA in nude mice was determined by PCR.The histopathology of tumor tissues was observed using with hematoxylin-eosin staining.The expressions of BMI-1,p-PI3K and p-Akt protein in nude mice were detected by immunohistochemistry and western blot.Results:Compared with the empty plasmid group,the proliferation ability of cells in the BMI-1- group was significantly decreased,and the proliferation ability of cells in the BMI-1+ group was significantly increased.Compared with the cells in the BMI-1+ group,the proliferation ability of the cells in the BMI-1+-Miltefosine group was significantly decreased.Compared with the empty plasmid group,the weight of nude mice in BMI-1--FOX-NY group was significantly reduced (P＜0.001),the tumor volume was obviously reduced (P＜0.001),the tumor histopathology was remarkally improved,the BMI-1 mRNA level,the BMI-1,p-PI3K and p-Akt protein levels were significantly reduced (P＜0.001).On the contrary,in BMI-1+-FOX-NY group,the weight of nude mice increased significantly (P＜0.01),the tumor volume increased prominently (P＜0.01,P＜0.001),the tumor histopathology deteriorated significantly,the BMI-1 mRNA level (P＜0.001),the BMI-1,p-PI3K,p-Akt protein level increased largely (P＜0.01,P＜0.001).Conclusion:BMI-1 expression is significantly increased in lymphoma,BMI-1 can promote the growth of DLBCL and activate the PI3K/Akt signaling pathway.

References:

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Memo

Memo:

云南省科技厅省基础研究计划(昆医联合专项)［编号：2019FE001(-276)］

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