|Table of Contents|

The effect of hydroxyapatite nanoparticles on the proliferation and apoptosis of osteosarcoma U2OS cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 04
Page:
530-533
Research Field:
Publishing date:

Info

Title:
The effect of hydroxyapatite nanoparticles on the proliferation and apoptosis of osteosarcoma U2OS cells
Author(s):
Wang Qian; Cao Jingjing; Wang Honggang; Chen Dafu; Wang Renxian
Beijing Jishuitan Hospital/the Institute of Traumatology and Orthopaedics,Beijing 100035,China.
Keywords:
hydroxyapatite nanoparticles; osteosarcoma; proliferation; apoptosis
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2020.04.002
Abstract:
Objective:To investigate the effect of nano-hydroxyapatite (Nano-HAP) on proliferation and apoptosis of human osteosarcoma U2OS cells.Methods:Human osteosarcoma cells U2OS were treated with Nano-HAP at different concentrations.The effect of nano-HAP on cell adhesion was detected by FDA staining.And the effect of Nano-HAP on cell proliferation was detected by CCK8 assay and plate clone formation.At last,the AnnexinⅤ-FITCapoptosis detection kit was used to detect the effect of Nano-HAP on cell apoptosis.Results:Different concentrations of Nano-HAP had no significant effect on the adhesion of U2OS,but had a significant inhibitory effect on the cloning formation,meanwhile,the proliferation of U2OS cells could be significantly inhibited at 50 μg/ml and 800 μg/ml.Conclusion:Nano-HAP could significantly inhibit the proliferation of osteosarcoma cell U2OS and promote cell apoptosis,however,it has no significant effect on the initial cell adhesion.

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Memo

Memo:
National Natural Science Foundation of China（No.51973021）;国家自然科学基金（编号：51973021）;北京市自然科学基金项目（编号：7192027）
Last Update: 2020-01-14