|Table of Contents|

Effect of miR-147a on the cell cycle and proliferation of bladder cancer cells by inhibiting the expression of CCND1 and CDK4

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 04
Page:
525-530
Research Field:
Publishing date:

Info

Title:
Effect of miR-147a on the cell cycle and proliferation of bladder cancer cells by inhibiting the expression of CCND1 and CDK4
Author(s):
Wang Zenghui; Zhang Xiaotong; Piao Chiyuan; Kong Chuize; Zhang Zhe
Department of Urology,First Affiliated Hospital of China Medical University,Liaoning Shenyang 110001,China.
Keywords:
bladder cancer; miR-147a; cell cycle; cell proliferation; CCND1; CDK4
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2020.04.001
Abstract:
Objective：To investigate the expression of miR-147a in bladder cancer tissues and cells,observe its effect on the proliferation and cell cycle of bladder cancer cells.To investigate the effect of miR-147a targeting negative regulation of CCND1 and CDK4 on the cell cycle and proliferation of bladder cancer cells.Methods：The expressions of miR-147a in normal bladder,bladder cancer tissues,normal bladder epithelial cells and bladder cancer cell lines were detected by qRT-PCR.The change of cell characteristics was observed by cells transfected with miR-147a mimics.The ability of cell proliferation was observed by CCK-8 assay.The cell cycle was observed by flow cytometry.Bioinformatics method analysed target genes of miR-147a,and GO gene classification and KEGG analysis of target genes found genes related to proliferation and cycle.Target genes of miR-147a were verified by western blot,using the dual luciferase reporter gene system to detect the effect of miR-147a on the transcriptional activities of CCND1 and CDK4.CCND1 and CDK4 specific siRNAs were used to observe their effects on cell cycle,using miR-147a inhibitor to replenish the experiment.Results：Compared with normal tissues/cells,miR-147a expression was significantly decreased in bladder cancer tissues/bladder cancer cell lines (P＜0.05).miR-147a can cause G1/S phase arrest and reduce cell proliferation in bladder cancer cells.Two target genes CCND1 and CDK4 related to cell proliferation and cell cycle were identified by a series of biosignal analysis.CCND1 and CDK4 were identified as target genes of miR-147a by indirect and direct experiments.The downregulation of CCND1 and CDK4 can cause G1/S phase arrest in bladder cancer cells,and inhibiting the expression of miR-147a can abolish G1/S phase arrest.Conclusion：miR-147a can be used as a tumor suppressor as a biomarker for the diagnosis of bladder cancer.miR-147a inhibits the G1/S phase of bladder cancer cells and inhibits the proliferation of bladder cancer cells by downregulating the expression of CCND1 and CDK4.

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Memo

Memo:
National Natural Science Foundation of China（No.81202000）;国家自然科学基金项目（编号：81202000）
Last Update: 2020-01-14