|Table of Contents|

Effect of AFAP1-AS1 expression on cell proliferation and apoptosis in silencing GCB type diffuse large B-cell lymphoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 02
Page:
210-213
Research Field:
Publishing date:

Info

Title:
Effect of AFAP1-AS1 expression on cell proliferation and apoptosis in silencing GCB type diffuse large B-cell lymphoma cells
Author(s):
Ling YipengZeng HuiPeng MiaoxinZhang CuilingChen Bing
Department of Hematology,Drum Tower Hospital,Medical College of Nanjing University,Jiangsu Nanjing 210008,China.
Keywords:
diffuse large B-cell lymphomaAFAP1-AS1proliferationapoptosis
PACS:
R733
DOI:
10.3969/j.issn.1672-4992.2020.02.007
Abstract:
Objective:To investigate the effect of AFAP1-AS1 expression on cell proliferation and apoptosis in diffuse large B cell lymphoma (DLBCL) with silent germinal center (GCB).Methods:Adenovirus samples were prepared.The expression of AFAP1-AS1 was required to be silenced,and OCI-Ly1 cell lines were transfected after the logarithmic growth of cultured GCB-DLBCL cells.The established GCB-DLBCL cell lines were required to silence the expression of AFAP1-AS1 through relevant operations.The experimental group was adenovirus infected cells.The sh-NC unrelated sequence adenovirus infected cells was the unrelated sequence control group,and the uninfected adenovirus cells were the blank group.The expression level of AFAP1-AS1 was detected by PCR.Cell proliferation was determined by CCK-8,and cell apoptosis was detected by flow cytometry.Results:AFAP1-AS1 expression level showed that the interference efficiency of the three shRNA sequences was stronger than that of the unrelated sequence control group (sh-NC),and the difference was statistically significant (P<0.05).Cell apoptosis in each group was detected by CCK-8 method.The results showed that after adenovirus sh3-AFAP1-AS1 infection,the absorbance of cells in OCI-Ly1 cell line was significantly lower than that in the control group and the blank group.The cell proliferation of GCB-DLBCL was inhibited by down-regulating AFAP1-AS1 (P<0.05).Flow cytometry was used to detect the apoptosis of cells in each group.The results showed that after sh3-AFAP1-AS1 and sh-NC transfection,the apoptosis rate of OCI-Ly1 cells in the experimental group was significantly higher than that in the unrelated sequence control group and the blank group,and the apoptosis of GCB-DLBCL cells could be induced by down-regulating AFAP1-AS1 (P<0.05).Conclusion:Silencing the expression of AFAP1-AS1 in GCB-DLBCL cells can effectively inhibit cell proliferation,induce apoptosis,or serve as a target for GCB-DLBCL therapy.

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Memo

Memo:
江苏省自然科学基金项目(编号:BK20140100)
Last Update: 2019-11-29