|Table of Contents|

Silencing MTA1 inhibits the adhesion,migration and invasion of hepatocellular carcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 02
Page:
214-219
Research Field:
Publishing date:

Info

Title:
Silencing MTA1 inhibits the adhesion,migration and invasion of hepatocellular carcinoma cells
Author(s):
Zhao Pengwei; Huang Jianzhao; Liu Yan; Liu Yan; Liu Jiangwei; Zhang Jiawei
Department of Hepatobiliary Surgery,Guizhou People's Hospital,Guizhou Guiyang 550002,China.
Keywords:
hepatoma cells; adhesion; migration; invasion; MTA1
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2020.02.008
Abstract:
Objective:To study the effect of silencing MTA1 on the adhesion,migration and invasion on hepatocellular carcinoma cells.Methods:MTA1 siRNA and siRNA control were transfected into hepatoma cells,at the same time,the cells without transfection were used as control.qRT-PCR and Western blot were used to detect the expression level of MTA1.MTT detection of cell proliferation,cell adhesion test was used to detect cell adhesion.Transwell was used to detect cell invasiveness and migration.Western blot was used to detect the levels of MMP-2,MMP-9,E-cadherin and N-cadherin protein in cells.Gelatinase zymography was used to detect the activity of MMP-2 and MMP-9.Results:Transfection of MTA1 siRNA in cells could downregulate the level of MTA1 mRNA and protein in hepatoma cells.Transfection of siRNA control had no effect on MTA1 mRNA and protein level in cells.After silencing MTA1,the survival rate and adhesion rate of hepatoma cells were decreased.The number of invasive cells was reduced.Compared with cells without transfection,the difference was statistically significant (P＜0.05).After silencing MTA1,the levels of MMP-2,MMP-9 protein and activity were decreased,at the same time,the level of E-cadherin protein was increased,and the level of N-cadherin protein was reduced.Compared with cells without transfection,the difference was statistically significant (P＜0.05).Conclusion:Silencing MTA1 inhibits the adhesion,migration and invasion of hepatocellular carcinoma cells.The mechanism is related to the inhibition of cell secretion of MMP-2,MMP-9 and inhibition of EMT.

References:

［1］Liu H,Huh YO.A case of multiple myeloma mimics:Extensive bone metastasis of hepatocellular carcinoma without liver mass［J］.Blood,2016,127(3):368.
［2］Winkler J,Roessler S,Sticht C,et al.Cellular apoptosis susceptibility (CAS) is linked to integrin β1 and required for tumor cell migration and invasion in hepatocellular carcinoma (HCC)［J］.Oncotarget,2016,7(16):22883-22892.
［3］Bing X,Jian L,Liu X,et al.TXNDC5 is a cervical tumor susceptibility gene that stimulates cell migration,vasculogenic mimicry and angiogenesis by down-regulating SERPINF1 and TRAF1 expression［J］.Oncotarget,2017,8(53):91009-91024.
［4］Fan C,Lin Y,Mao Y,et al.MicroRNA-543 suppresses colorectal cancer growth and metastasis by targeting KRAS,MTA1 and HMGA2［J］.Oncotarget,2016,7(16):21825-21839.
［5］Zhong LI,Zhao HQ.Expression of MTA1 in different cell lines and liver cancer tissues［J］.Pharmaceutical Biotechnology,2011,18(2):106-110.
［6］TIAN J,XIAO HT,FENG H,et al.Expression of metastasis-associated gene 1 in ovarian cancer and its influence on the invasion and metastasis of ovarian cancer cells［J］.Chin J Clin Oncol,2012,39(21):1619-1622.［田菁,肖会廷,冯慧,等.MTA1在卵巢癌中的表达及其对卵巢癌细胞侵袭转移影响的研究［J］.中国肿瘤临床,2012,39(21):1619-1622.］
［7］Hofer MD,Chang MC,Hirko KA,et al.Immunohistochemical and clinicopathological correlation of the metastasis-associated gene 1 (MTA1) expression in benign and malignant pancreatic endocrine tumors［J］.Mod Pathol,2009,22(7):933-939.
［8］Toh Y,Kuninaka S,Endo K,et al.Molecular analysis of a candidate metastasis-associated gene,MTA1:Possible interaction with histone deacetylase 1［J］.J Exp Clin Cancer Res,2000,19(1):105-111.
［9］Khaleque MA,Bharti A,Gong J,et al.Heat shock factor 1 represses estrogen-dependent transcription through association with MTA1［J］.Oncogene,2008,27(13):1886-1893.
［10］Zhu W,Cai MY,Tong ZT,et al.Overexpression of EIF5A2 promotes colorectal carcinoma cell aggressiveness by upregulating MTA1 through C-myc to induce epithelial-mesenchymaltransition［J］.Gut,2012,61(4):562-575.
［11］Li K,Wang J,Ivanovic M,et al.Targeting prostate cancer angiogenesis through metastasis-associated protein 1 (MTA1)［J］.Prostate,2011,71(3):268-280.
［12］Martin MD,Fischbach K,Osborne CK,et al.Loss of heterozygosity events impeding breast cancer metastasis contain the MTA1 gene［J］.Cancer Research,2013,61(9):3578-3580.
［13］Dannenmann C,Shabani N,Friese K,et al.The metastasis-associated gene MTA1 is upregulated in advanced ovarian cancer,represses ERbeta,and enhances expression of oncogenic cytokine GRO［J］.Cancer Biology & Therapy,2008,7(9):1460-1467.
［14］Murakami M,Kaul R,Robertson ES.MTA1 expression is linked to ovarian cancer［J］.Cancer Biology & Therapy,2008,7(9):1468-1470.
［15］Gururaj AE,Singh RR,Rayala SK,et al.MTA1,a transcriptional activator of breast cancer amplified sequence 3［J］.Proceedings of the National Academy of Sciences of the United States of America,2006,103(17):6670-6675.
［16］YANG JW.Expression and significance of MTA1 in liver cancer［J］.Chongqing Med,2012,41(3):264-265.［杨建伟.MTA1蛋白在肝癌组织中的表达及意义［J］.重庆医学,2012,41(3):264-265.］
［17］Contursi A,Sacco A,Grande R,et al.Platelets as crucial partners for tumor metastasis:From mechanistic aspects to pharmacological targeting［J］.Cellular & Molecular Life Sciences,2017,74(10):1-17.
［18］Yeh CM,Lin CW,Yang JS,et al.Melatonin inhibits TPA-induced oral cancer cell migration by suppressing matrix metalloproteinase-9 activation through the histone acetylation［J］.Oncotarget,2016,7(16):21952-21967.
［19］Zhou B,Wang Y,Jiang J,et al.The long non-coding RNA colon cancer-associated transcript-1/miR-490 axis regulates gastric cancer cell migration by targeting hnRNPA1［J］.Iubmb Life,2016,68(3):201-210.
［20］Fernandes S,Salta S,Bravo J,et al.Acetyl-L-carnitine prevents methamphetamine-induced structural damage on endothelial cells via ILK-related MMP-9 activity［J］.Molecular Neurobiology,2016,53(1):408-422.
［21］Yang C,Zhang LJ,Wu FX,et al.Expressions and relationship between MTA1 and MMP-2 in hepetocellular carcinoma tissues［J］.Chinese Journal of Cancer Prevention & Treatment,2010,18(9):693-696.
［22］Krebs AM,Mitschke J,Lasierra LM,et al.The EMT-activator ZEB1 is a key factor for cell plasticity and promotes metastasis in pancreatic cancer［J］.Nature Cell Biology,2017,19(5):518-529.
［23］Yao L,Zhang D,Zhao X,et al.Dickkopf-1-promoted vasculogenic mimicry in non-small cell lung cancer is associated with EMT and development of a cancer stem-like cell phenotype［J］.Journal of Cellular & Molecular Medicine,2016,20(9):1673-1685.
［24］Ravi J,Elbaz M,Wani NA,et al.Cannabinoid receptor-2 agonist inhibits macrophage induced EMT in non-small cell lung cancer by downregulation of EGFR pathway［J］.Mol Carcinog,2016,55(12):2063-2076.
［25］PANG C，ZHANG J，LIU WC.The research progression of EMT［J］.Modern Oncology,2016,24(15):2484-2487.［庞翠,张菊,刘文超.EMT研究进展［J］.现代肿瘤医学,2016,24(15):2484-2487.］
［26］Ma K,Fan Y,Dong X,et al.MTA1 promotes epithelial to mesenchymal transition and metastasis in non-small cell lung cancer［J］.Oncotarget,2017,8(24):38825-38840.

Memo

Memo:
-
Last Update: 2019-11-29