|Table of Contents|

Expression and significance of miR-34c-3p and M2 type of tumor associated macrophage in triple negative breast cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 01
Page:
4-8
Research Field:
Publishing date:

Info

Title:
Expression and significance of miR-34c-3p and M2 type of tumor associated macrophage in triple negative breast cancer
Author(s):
Shi Wenglong1Wu Jiang1Meng Qingjie1Ye Biaofei2Li Nanlin1Ling Rui1Chen Jianghao1
1.Department of Thyroid Breast and Vascular Surgery,Xijing Hospital,Air Force Medical University,Shaanxi Xi'an 710032,China; 2.Department of Oncology,Tangdu Hospital,Air Force Medical University,Shaanxi Xi'an 710038,China.
Keywords:
miR-34c-3pM2 type of TAMIL-10TNBC
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2019.01.002
Abstract:
Objective:To detect the expression of miR-34c-3p and M2 type of tumor-associated macrophage (TAM) in triple negative breast cancer (TNBC) patients and explore their significance in the pathogenesis of breast cancer.Methods:All 68 TNBC patients in Xijing Hospital from February 2017 to January 2018 were selected as the experimental group (group A),and 70 breast fibroadenoma patients in the same period were treated as the control group (group B).Real-time RT-PCR was used to detect the expression of miR-34c-3p.M2 type of TAM expression was detected by flow cytometry.The expression of IL-10 in serum was detected by ELISA.To analyze of the correlation between miR-34c-3p and M2 type of TAM and IL-10.Results:miR-34c-3p in group A was significantly lower than that in group B,and the relative expression level was 0.84±0.08,1.29±0.71 respectively,and the difference was statistically significant (P<0.05).Compared with group B,the proportion of M2 type of TAM in group A (CD68+CD163+) was increased significantly (P<0.05).The expression of IL-10 in group A [(19.69±4.93) pg/ml] was significantly higher than that in group B [(17.26±3.51) pg/ml](P<0.05).The expression of miR-34c-3p in TNBC tissues was negatively correlated with the ratio of M2 type of TAM and serum IL-10 level (r=-0.508,r=-0.656,P<0.05).Conclusion:The expression of miR-34c-3p in TNBC tissues was down-regulated,and the proportion of M2 type of TAM and serum IL-10 expression were significantly elevated,which contributed to the progression of TNBC.

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Memo

Memo:
National Natural Science Foundation of China(No.81472598);国家自然科学基金项目(编号:81472598);陕西省社会发展科技公关项目(编号:2016SF-298)
Last Update: 2018-11-30