|Table of Contents|

The expression and clinical significance of LINC01535 in epithelial ovarian cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 10
Page:
1887-1892
Research Field:
Publishing date:

Info

Title:
The expression and clinical significance of LINC01535 in epithelial ovarian cancer
Author(s):
WANG Juan; HAO Yali; LIU Leilei; KANG Shan
The Fourth Hospital of Hebei Medical University，Hebei Shijiazhuang 050011,China.
Keywords:
epithelial ovarian cancer; LINC01535; prognosis; proliferation; migration; invasion; EMT
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2024.10.022
Abstract:
Objective：To detect the expression of LINC01535 gene in epithelial ovarian cancer (EOC) tissues and cell lines,as well as its relationship with clinical pathology and prognosis of patients.To explore the impact of LINC01535 on the proliferation,invasion,and EMT of epithelial ovarian cancer cell line CAOV3.Methods:Based on the GEPIA2 and Kaplan-Meier Plotter databases,the expression of LINC01535 in epithelial ovarian cancer tissue and its impact on survival of ovarian cancer patients were analyzed.The real-time fluorescence quantitative qRT-PCR method was used to detect the expression level of LINC01535 gene in epithelial ovarian cancer tissue,normal tissue,and three epithelial ovarian cancer cell lines (SKOV3,A2780,and CAOV3).The relationship between LINC01535 gene expression and clinical pathological parameters and prognosis was analyzed.After using siRNA transient transfection to interfere with the expression of LINC01535 gene in epithelial ovarian cancer cells CAOV3,MTS and Transwell cell experiments were performed to detect the impact of abnormal expression of LINC01535 gene on the proliferation,migration,and invasion of epithelial ovarian cancer cell CAOV3.The effect of LINC01535 on the expression levels of EMT process related biomarkers (E-cadherin,N-cadherin,and Vimentin) was detected using qRT-PCR.Results:Using GEPIA2 and Kaplan-Meier Plotter databases,it was found that the expression of LINC01535 in epithelial ovarian cancer tumor tissue was significantly higher than that in normal tissue (P＜0.05),and the overall survival rate of the high expression group of LINC01535 was significantly lower than that of the low expression group of LINC01535 (P＜0.01).The expression of LINC01535 gene in 89 cases of epithelial ovarian cancer tissue was significantly higher than that of normal tissue (P＜0.01),and was associated with greater omentum metastasis,lymph node metastasis,FIGO staging,and survival (P＜0.01 or P＜0.05).Knocking down LINC01535 gene can inhibit the in vitro proliferation,migration,invasion ability and EMT process of epithelial ovarian cancer cell line CAOV3 (P＜0.01 or P＜0.05).Conclusion:LINC01535 gene may play a role as an oncogene in epithelial ovarian cancer.Its high expression may promote the proliferation,invasion,and EMT process of epithelial ovarian cancer cells,and may become a candidate molecular marker for prognostic evaluation of epithelial ovarian cancer patients.

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Memo

Memo:
河北省自然科学基金重点项目（编号：H2020206385,H2020206651）
Last Update: 1900-01-01