|Table of Contents|

The anticancer effects and mechanism of Irisin and Irisin-liposomes in non-small cell lung cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 10
Page:
1792-1798
Research Field:
Publishing date:

Info

Title:
The anticancer effects and mechanism of Irisin and Irisin-liposomes in non-small cell lung cancer
Author(s):
YU Zhihui1; YUAN Bin2; ZHUGE Xiangzhen3; YU Xiangyuan4
1.Department of Respiratory and Critical Care Medicine,Nanxishan Hospital of Guangxi Zhuang Autonomous Region,Guangxi Guilin 541000,China;2.Department of Respiratory and Critical Care Medicine,Miluo People's Hospital,Hunan Miluo 414400,China;3.Department of Clinical Nutrition,Affiliated Hospital of Guilin Medical University,Guangxi Guilin 541000,China;4.School of Public Health,Guilin Medical University,Guangxi Guilin 541000,China.
Keywords:
Irisin; liposomes; non-small cell lung cancer; ferroptosis; ROS
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.10.005
Abstract:
Objective：To investigate the effects of Irisin and Irisin-liposomes (LPs) on human non-small cell lung cancer H1299 cells and their potential mechanisms of action.Methods:Nanoparticle characterization and cellular mitochondrial morphology were observed by transmission electron microscopy.CCK8 and flow cytometry were used to analyze the effects of Irisin and Irisin-LPs on cell viability,apoptosis,and cycling of H1299 cells.qRT-PCR and Western blot were used to analyze the effects of Irisin on Nrf2/HO-1 signaling.Results:Irisin and Irisin-LPs significantly inhibited the growth and cell cycle of H1299 cells and promoted their apoptosis,with Irisin-LPs having a more pronounced effect.Irisin and Irisin-LPs significantly altered cytoskeletal structures and mitochondrial functions as revealed by cytoskeleton staining and mitochondrial membrane potential detection.Furthermore,Irisin and Irisin-LPs significantly induced iron-dependent lipid peroxidation,i.e.,ferroptosis.In addition,Irisin and Irisin-LPs significantly regulated the expression of the Nrf2/HO-1 signaling pathway.Conclusion:This study reveals the anti-lung cancer activity and potential mechanisms of Irisin and Irisin-LPs,providing new theoretical and experimental evidence for the clinical application of Irisin.

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