|Table of Contents|

The effect of high expression of SNORA73B on stem cell-like characteristics in laryngeal squamous cell carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 10
Page:
1799-1804
Research Field:
Publishing date:

Info

Title:
The effect of high expression of SNORA73B on stem cell-like characteristics in laryngeal squamous cell carcinoma
Author(s):
WANG Jingtian1; ZHAO Yan1; WU Ganxun1; LIU Shenghui1; LAN Lili1; HU Guobin1; XU Yuru1; HAO Kaikai1; SHEN Supeng2
1.Otorhinolaryngology Head and Neck Surgery,the Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050011,China;2.Biological Specimen Library,Hebei Provincial Cancer Institute,the Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050011,China.
Keywords:
small nucleolar RNA; SNORA73B; laryngeal squamous cell carcinoma; prognosis; cancer stem cell
PACS:
R739.65
DOI:
10.3969/j.issn.1672-4992.2024.10.006
Abstract:
Objective：To investigate the potential role of small nucleolar RNA 73B (SNORA73B) in laryngeal squamous cell carcinoma (LSCC) by examining its high expression and clinical significance,to explore how SNORA73B may contribute to the proliferation,migration,and invasion of LSCC cells by maintaining stem cell characteristics.Methods:Quantitative PCR (qPCR) was employed to measure the expression level of SNORA73B in LSCC tissues and cells.Furthermore,in vitro cultured cells were utilized for MTS assays,scratch healing assays,and Transwell experiments to assess the impact of SNORA73B knockdown or overexpression on cell proliferation,migration,and invasion.Additionally,the role of SNORA73B in influencing the stem cell-like characteristics of LSCC cells was evaluated using a tumor cell spheroidization assay.Results:The results obtained from qPCR analysis demonstrated a substantial increase in the expression of SNORA73B in both LSCC tissues and cells compared to the corresponding adjacent cancer tissues and control group (P＜0.05).This elevated expression was found to be associated with pathological grading,lymph node metastasis,and poor prognosis in patients (P＜0.05).Moreover,overexpression of SNORA73B in Tu177 cells notably enhanced their proliferation,scratch healing,migration,and invasion capabilities(P＜0.05).Conversely,interference with SNORA73B led to a significant reduction in these cellular functions(P＜0.05).To further investigate the impact of SNORA73B on the development of LSCC stem cell-like characteristics,laryngeal cancer spheroid cells were induced(P＜0.05).The induction resulted in a notable increase in the expression of tumor stem cell markers OCT4 and SOX2 proteins (P＜0.05).Additionally,SNORA73B expression was significantly upregulated (P＜0.05).However,interference with SNORA73B led to a significant decrease in the number of cell spheroids(P＜0.05) as well as reduced mRNA and protein expressions of OCT4 and SOX2.Conclusion:The high expression of SNORA73B appears to be associated with the development and malignant progression of LSCC.It is likely that SNORA73B plays a role in mediating stem cell-like characteristics in LSCC cells,leading to increased tumor cell proliferation and migration.

References:

［1］ JOHNSON DE,BURTNESS B,LEEMANS CR,et al.Head and neck squamous cell carcinoma［J］.Nat Rev Dis Primers,2020,6(1):92.
［2］ LI DP,SUN AJ,ZHANG L,et al.Elevated ITGA5 facilitates hyperactivated mTORC1-mediated progression of laryngeal squamous cell carcinoma via upregulation of EFNB2［J］.Theranostics,2022,12(17):7431-7449.
［3］WANG J,WU YY,GAO W,et al.Identification and characterization of CD133(+)CD44(+) cancer stem cells from human laryngeal squamous cell carcinoma cell lines［J］.J Cancer,2017,8(3):497-506.
［4］YUAN SS,WEI SS,XIANG XH,et al.SNORA42 promotes oesophageal squamous cell carcinoma development through triggering the DHX9/p65 axis［J］.Genomics,2021,113(5):3015-3029.
［5］ZOU AE,KU JJ,HONDA TK,et al.Transcriptome sequencing uncovers novel long noncoding and small nucleolar RNAs dysregulated in head and neck squamous cell carcinoma［J］.RNA,2015,21(6):1122-1134.
［6］WOLF J,CLAUDIA AH,SCHLECHT A,et al.Transcriptional characterization of conjunctival melanoma identifies the cellular tumor microenvironment and prognostic gene signatures ［J］.Sci Rep,2020,10(1):17022.
［7］ BATLLE E,CLEVERS H.Cancer stem cells revisited［J］.Nat Med,2017,23(10):1124-1134.
［8］ WALCHER L,KISTENMACHER AK,SUO H,et al.Cancer stem cells-origins and biomarkers:perspectives for targeted personalized therapies［J］.Front Immunol,2020,11:1280.
［9］LIANG JN,WEN JY,HUANG Z,et al.Small nucleolar RNAs:Insight into their function in cancer［J］.Front Oncol,2019,9:587.
［10］CAO PB,YANG AQ,WANG R,et al.Germline duplication of SNORA18L5 increases risk for HBV-related hepatocellular carcinoma by altering localization of ribosomal proteins and decreasing levels of p53［J］.Gastroenterology,2018,155(2):542-556.
［11］WAJAHAT M,BRACKEN CP,ORANG A.Emerging functions for snoRNAs and snoRNA-derived fragments［J］.Int J Mol Sci,2021,22(19):10193.
［12］LIN YL,HOLDEN V,DHILIPKANAH P,et al.A non-coding RNA landscape of bronchial epitheliums of lung cancer patients［J］.Biomedicines,2020,8(4):88.
［13］ CHEN X,LI QH,XIE BM,et al.SNORA73B promotes endometrial cancer progression through targeting MIB1 and regulating host gene RCC1 alternative splicing［J］.J Cell Mol Med,2023，27(19):2890-2905.
［14］ JAFARE S,RAVAN M,SANI IK,et al.Screening and identification of potential biomarkers for pancreatic cancer:An integrated bioinformatics analysis［J］.Pathol Res Pract,2023,249:154726.
［15］ HAN C,SUN LY,LUO XQ,et al.Chromatin-associated orphan snoRNA regulates DNA damage-mediated differentiation via a non-canonical complex［J］.Cell Rep,2022,8(13):110421.

Memo

Memo:
河北省重点研发计划项目（编号：22377736D,22377721D）；河北省自然科学基金项目（编号：H2022206326）
Last Update: 1900-01-01