|Table of Contents|

ABCA8 inhibits the proliferation,migration,and invasion of colorectal cancer cells by regulating Wnt/β-catenin signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 10
Page:
1781-1786
Research Field:
Publishing date:

Info

Title:
ABCA8 inhibits the proliferation,migration,and invasion of colorectal cancer cells by regulating Wnt/β-catenin signaling pathway
Author(s):
PENG Su; YANG Lebin; SHAN Hanguo
Department of Gastrointestinal Surgery,the Second Affiliated Hospital,Hengyang Medical School,University of South China,Hunan Hengyang 421001,China.
Keywords:
colorectal cancer; ATP binding cassette subfamily A member 8; cell proliferation; cell migration; cell invasion; Wnt/β-catenin signaling pathway
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2024.10.003
Abstract:
Objective：To study the effect of ATP binding cassette subfamily A member 8 (ABCA8) on the proliferation,migration,and invasion of colorectal cancer (CRC) cells,and to clarify its mechanism.Methods:The expression of ABCA8 in CRC tissues was analyzed using bioinformatics,and real-time fluorescence quantitative PCR (RT-qPCR) was used to detect the expression level of ABCA8 mRNA in clinical CRC cancer tissues and various CRC cell lines.SW480 cells were infected with the overexpression of ABCA8 lentivirus,and then divided into Blank group,Vector group,ABCA8 overexpression (Oe-ABCA8) group,Vector+HLY78 group,and Oe-ABCA8+HLY78 group.The intervention concentration of the Wnt/β-catenin signaling pathway agonist HLY78 was 10 μmol/L.CCK-8 assay was used to detect cell proliferation activity in each group.Transwell assay was used to detect the number of cell migration and invasion in each group.Western blot method was uesd to detect the protein expression levels of ABCA8,β-catenin,c-Myc,and metalloproteinase-9 (MMP-9).Results:ABCA8 showed low expression levels in CRC cancer tissue and CRC cell lines SW480,SW620,HCT116,and HT-29.Compared with the Blank group,the proliferation activity of SW480 cells in the oe-ABCA8 group was significantly reduced (P＜0.05),the number of migrating and invading cells was significantly reduced (P＜0.05),and the expression of ABCA8 in cells was significantly increased (P＜0.05).The protein expression levels of β-catenin,c-Myc,and MMP-9 were significantly reduced (P＜0.05).However,there was no significant difference in all indicators in the Vector group.Compared with the Vector group,the proliferation activity of SW480 cells in the Vector+HLY78 group were significantly increased (P＜0.05),and the number of migrating and invading cells was significantly increased (P＜0.05).The protein expression levels of β-catenin,c-Myc,and MMP-9 were significantly increased (P＜0.05).Compared with the Oe-ABCA8 group,the proliferation activity of SW480 cells in the Oe-ABCA8+HLY78 group were significantly increased (P＜0.05),and the number of migrating and invading cells was significantly increased (P＜0.05).The protein expression levels of β-catenin,c-Myc,and MMP-9 were significantly increased (P＜0.05).Conclusion:ABCA8 is low expressed in CRC tissues and cell lines,and overexpression of ABCA8 can inhibit the proliferation,migration,and invasion of CRC cell line SW480.Its mechanism may be related to the inhibition of Wnt/β-catenin signaling.

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Last Update: 1900-01-01