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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2024 10

Page:

1767-1773

Research Field:

Publishing date:

Info

Title:

Xiaoliuteng ethyl acetate extraction' s impact on proliferation,apoptosis,and the Bax/Bcl-2/Caspase-3 signaling pathway in human colorectal cancer cells

Author(s):

ZHOU Huansi¹; LI Yanping²; JIN Mingjing²; ZHAO Xiangpei²; ZHAO Yuqian²; LU Chunhua³

1.Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine,Guangxi Nanning 530000,China;2.Research Laboratory of Zhuang & Yao Medicine,Guangxi International Zhuang Medicine Hospital,Guangxi Nanning 530000,China;3.Medical Experimental Center,the First People's Hospital of Nanning,Guangxi Nanning 530021,China.

Keywords:

Pileostegia tomentella; colorectal cancer; cell cycle; mitochondrial membrane potential; apoptosis

PACS:

R735.3

DOI:

10.3969/j.issn.1672-4992.2024.10.001

Abstract:

Objective：To investigate the effects of Xiaoliuteng ethyl acetate extraction (XLTE) on human colorectal cancer cells (HCT116 and HT29),with a focus on its impact on cell proliferation,apoptosis,and the cell cycle,and to explore its relationship with Bax/Bcl-2/Caspase-3 signaling pathway.Methods:The morphological changes of HCT116 and HT29 cells under XLTE were monitored by Incucyte S3 live cell dynamic analysis system and IC50 values of 48 h were fitted.The proliferation ability of HCT116 and HT29 cells was detected by CCK-8 assay,colony formation assay and cell cycle assay.The proapoptotic ability of HCT116 and HT29 cells was detected by Hoechst 33258 staining,Rhodamine 123 staining and the expressions of Bax,Bcl-2 and Caspase-3 proteins were detected by Western blot.Results:XLTE exhibited significant inhibitory effects on the proliferation of HCT116 and HT29 cells,with 48 h's IC50 values of 567 μg/mL and 549 μg/mL,respectively.In addition,XLTE was found to arrest the cell cycle in the G0/G1 phase,promote apoptosis,induce chromatin contraction and breakage,and reduce mitochondrial membrane potential.Furthermore,XLTE was found to increase the expression of Bax and Caspase-3 protein,while decreasing the expression of Bcl-2 protein in the cells.Conclusion:XLTE was found to inhibit proliferation and promote apoptosis in HCT116 and HT29 cells of human colorectal cancer,which was related to the blocking of the cell cycle in the G0/G1 phase and the activation of the Bax/Bcl-2/Caspase-3 pathway.

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Memo

Memo:

广西自然科学基金（编号：2018GXNSFDA050009）；广西中医药大学博士启动基金（编号：2020BS034）；广西中医药大学附属国际壮医医院人才引进科研启动基金（编号：GZ2021RC016）

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Last Update: 1900-01-01