|Table of Contents|

The effects of Catalpol on proliferation,apoptosis and immune escape of lung cancer cells by regulating the cGAS-STING signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 08
Page:
1417-1423
Research Field:
Publishing date:

Info

Title:
The effects of Catalpol on proliferation,apoptosis and immune escape of lung cancer cells by regulating the cGAS-STING signaling pathway
Author(s):
ZHENG Liang; LIU Shuang; GUO Ran; YANG Wenping
Department of Respiratory and Critical Care Medicine，Jinan City People's Hospital，Shandong Jinan 271100，China.
Keywords:
Catalpol; GMP-AMP synthase/stimulator of interferon gene signaling pathway; lung cancer; proliferation; apoptosis; immune escape
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.08.008
Abstract:
Objective:To investigate the effects of Catalpol on the proliferation,apoptosis and immune escape of lung cancer cells by regulating the GMP-AMP synthase/stimulator of interferon gene (cGAS-STING) signaling pathway.Methods:Lung cancer cells A-427 were cultured and treated with 1~20 μmol/L Catalpol to select the optimal drug concentration.Cells were divided into Control group,Catalpol low concentration group (Catalpol-L group,5 μmol/L Catalpol),Catalpol medium concentration group (Catalpol-M group,10 μmol/L Catalpol),Catalpol high concentration group (Catalpol-H group,15 μmol/L Catalpol),and Catalpol high concentration+cGAS pathway inhibitor RU.521 group (Catalpol-H+RU.521 group,15 μmol/L Catalpol+1 μmol/L RU.521).MTT method was applied to detect cell survival rate.Edu method was applied to detect cell proliferation.Plate clone formation experiment was applied to detect cell clone formation.Flow cytometry was applied to detect cell apoptosis and cell cycle.Immunoblotting was applied to detect the expression of Bax,Bcl-2,Cleaved-caspase-3,cGAS,and STING proteins.CD8+T cells were co-cultured with cells treated in each group.Trypan blue staining was applied to detect the survival rate of CD8+T cells.ELISA kit was applied to detect levels of IFN-γ,IL-4,IL-10,TGF-β and PD-L1.Results:The survival rate of A-427 cells decreased after treatment with 1~20 μmol/L Catalpol (P＜0.05),therefore,concentrations of 5 μmol/L,10 μmol/L and 15 μmol/L Catalpol were selected for the experiment.Compared with the Control group,the positive rate of Edu,the number of cell clones formed,the proportion of S phase cells,the levels of IL-4,IL-10,TGF-β,PD-L1,and the protein level of Bcl-2 in the Catalpol-L,Catalpol-M,and Catalpol-H groups were obviously reduced (P＜0.05),the rate of cell apoptosis,the proportions of G0/G1 and G2/M phase cells,the survival rate of CD8+T cells,the level of IFN-γ,and the protein levels of Bax,Cleaved-caspase-3,cGAS and STING were obviously increased (P＜0.05),which were concentration dependent.Compared with the Catalpol-H group,the positive rate of Edu,the number of cell clones formed,the proportion of S phase cells,the levels of IL-4,IL-10,TGF-β,PD-L1,and the protein level of Bcl-2 in the Catalpol-H+RU.521 group were obviously increased (P＜0.05),the rate of cell apoptosis,the proportions of G0/G1 and G2/M phase cells,the survival rate of CD8+T cells,the level of IFN-γ,and the protein levels of Bax,Cleaved-caspase-3,cGAS,and STING were obviously reduced (P＜0.05).Conclusion:Catalpol might inhibit the proliferation and immune escape of lung cancer cells and promote cell apoptosis by activating the cGAS-STING signaling pathway.

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